Enzymatic determination of glucose

Details Enzymatic determination of glucose Enzymatic determination of glucose

1973-09-10

1976-06-22

Naff, David M.

Textiles: fiber preparation

195 63, 195 99, 1951035R, G01N 3114, C07G 702

Patent

active

039649741

ABSTRACT:
Glucose is rapidly ad quantitatively determined with an analytical agent containing glucose dehydrogenase having an activity of at least 2 .mu./mg and NADH.sub.2 oxidase activity less than 0.1%, a pyridine coenzyme, a buffer and mutarotase to increase spontaneous mutarotation of alpha-glucose to beta-glucose. There may be optionally present inhibitors for reduced pyridine coenzyme oxidases and a thermal stabilizing amount of alkali metal chloride.

REFERENCES:
patent: 3290228 (1966-12-01), Gretton et al.
patent: 3335069 (1967-08-01), Wachter
patent: 3413198 (1968-11-01), Deutsch
patent: 3703591 (1972-11-01), Bucolo et al.
patent: 3764478 (1973-10-01), Bergmeyer et al.
Miwa, et al., Matarotase Effect on Colorimetric Determination of Blood Glucose With B-D-Glucose Oxidase, Clin. Chim. Acta, vol. 37, Mar. 1972, (pp. 538-540).
Metzger, et al., Subcellular Distribution and Properties of Hepatic Glucose Dehydrogenase of Selected Vertebrates, J. of Biological Chemistry, vol. 240, No. 7, 1965, (pp. 2767-2771).
Sadoff, et al., Significance of Multiple Forms of Glucose Dehydrogenase in Relation to Its Heat Resistance, The Spores, III, 1965 (pp. 97-110).

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