Enzymatic determination of analyte ions in fluids by optimizing

Details Enzymatic determination of analyte ions in fluids by optimizing Enzymatic determination of analyte ions in fluids by optimizing

1992-06-22

1995-01-10

Witshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 77, 435 772, 435962, 435 18, 435 25, C12Q 140, C12Q 100, C12Q 134, C12Q 126

Patent

active

053806492

ABSTRACT:
A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium, magnesium, manganese, lithium, lead, zinc, copper, iron or other heavy metals or non-metallic ions comprising chloride, bicarbonate, protons, ammonium and substances that give rise to ammonium. The enzymes which are used may be a transferase, a hydrolase, an oxidoreductase or a lyase. An essential feature is a method to exclude interferences by ions by masking the interfering ions with a binding agent.

REFERENCES:
patent: 4657854 (1987-04-01), Wegfahrt
patent: 4734375 (1988-03-01), Charlton
patent: 4812400 (1989-03-01), Steinman
Outlaw W. H., Measurement of 10.sup.-7 -10.sup.-12 Mol of Potassium . . . Anal Biochem 92, 370-374 1979.
Wimmer M. C., A Kinetic Colorimetric Procedure for Quantifying Mg in Serum, Clin Chem 32/4 629-632 1986.

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