Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – Enzyme component of specific activity or source
Patent
1997-04-24
1999-07-27
Fries, Kery
Cleaning compositions for solid surfaces, auxiliary compositions
Cleaning compositions or processes of preparing
Enzyme component of specific activity or source
510530, 510320, 510305, 510306, 435198, 435912, C11D 3386
Patent
active
059290170
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
This invention relates to an enzymatic detergent composition and an enzymatic detergent additive comprising a lipolytic enzyme.
BACKGROUND ART
Lipolytic enzymes are known to be useful in detergents to improve the removal of fatty stains. Thus, in recent years Lipolase.RTM., a microbial lipase derived from the fungus Thermomyces lanuginosus (also called Humicola lanuginosa), has been introduced into many commercial brands of detergent.
Other microbial lipases have also been suggested for use in detergents, e.g. bacterial lipase from Pseudomonas cepacia (U.S. Pat. No. 4,876,024), from Streptomycetes (WO 94/14940) and from Gongronella butleri strain NRRL 3521 (U.S. Pat. No. 3,634,195, the strain was previously named Absidia butleri, see K. H. Domsch et al., Compendium of Soil Fungi, Academic Press 1980, p. 381).
Many detergents are alkaline with a high pH in solution (e.g. around pH 10) and contain a builder to bind Ca.sup.++ ions, so there is a need for lipolytic enzymes with high activity at high pH in the absence of Ca.sup.++.
SUMMARY OF THE INVENTION
Surprisingly, we have found that a lipolytic enzyme with high activity at alkaline pH in the absence of Ca.sup.++ can be obtained from strains of filamentous fungi belonging to the genus Absidia and that the lipolytic enzymes are effective for improving the effect of detergents.
Accordingly, the invention provides an enzymatic detergent composition comprising a surfactant and an alkaline Absidia lipolytic enzyme. The invention also provides a method for removing fatty soiling from textile, comprising washing the textile in an aqueous solution comprising the detergent composition.
The invention further provides an enzymatic detergent additive containing an Absidia lipolytic enzyme as an active component, provided in the form of a non-dusting granulate, a stabilized liquid, a slurry, or a protected enzyme.
Another aspect of the invention provides a lipolytic enzyme which is derived from a strain of Absidia reflexa and has a higher lipolytic enzyme activity at pH 10 than at pH 9 in the absence of Ca.sup.++.
U.S. Pat. No. 3,634,195 describes production of lipase from A. cylindrospora var. rhizomorpha NRRL 2815 and A. blakesleeana NRRL 1305. S. Koritala et al., J. Am. Oil Chem. Soc., 64 (4), 509-13 (1987) discloses that soybean oil was partially hydrolyzed when incubated with A. coerula NRRL 5926 and A. ramosa NRRL 1309. T. Satyanarayana, Current Science, 50 (15), 680-2 (1981) discloses the secretion of lipase by a strain of A. corymbifera. K. Aisaka et al., Agric. Biol. Chem., 43 (10), 2125-2129 (1979) describes the formation of a lipoprotein lipase from Absidia hyalospora strain KY 303 (now classified as A. blakesleeana).
However, the prior art does not disclose or suggest that lipolytic enzymes from Absidia are active at high pH in the absence of Ca.sup.++, nor that they are useful in detergents.
BRIEF DESCRIPTION OF THE DRAWINGS
FIGS. 1-5 show graphs of lipolytic enzyme activity versus pH in the absence of Ca.sup.++ for some purified lipolytic enzymes according to the invention. Details are given in Example 8.
DETAILED DISCLOSURE OF THE INVENTION
The microbial strain used in this invention belongs to the genus Absidia, as described in M. A. A. Schipper, Persoonia, Vol. 14, Part 2, pp. 133-148 (1990). Within this genus, the following subgenera, groups, species and strains are preferred. Variants and mutants thereof capable of producing lipolytic enzyme may also be used in the invention. It is noted that a number of previously recognized species names were reclassified by Schipper, Op. cit., and for convenience the previously used names of some strains are also listed below.
The prior art does not describe lipolytic enzyme production from A. reflexa, a species which was not classified by Schipper. The production of a lipolytic enzyme by this species has not previously been described, and we have found that the lipolytic enzymes from this species is distinct from the lipolytic enzymes from the subgenera Mycocladus and Absidia.
_____
REFERENCES:
patent: 3634195 (1972-01-01), Melachouris
Chemical Abstracts: 95:111511y "Lipolytic Activity of Thermophilic Fungi of Paddy Straw Compost", 1981.
EMBL, Genbank, DDBJ, Accession No. A34959.
Satyanarayana, T., et al., Chemical Abstracts, vol. 95, No. 13, pp. 344 (1981) Abstract No. 111511y.
Dialog Information Services, file 351, Derwent WPI, Dialog accession No. 008384112, WPI accession No. 90-271113/36.
Koritala, S., et al., JAOCS, vol. 64, No. 4, pp. 509-513, (1987).
Aisaka, K., et al., Agric. Biol. Chem., vol. 43, No. 10, pp. 2125-2129 (1979).
Schipper, M.A.A., Persoonia, vol. 14, Part 2, pp. 133-148, (1990).
Abo Masanobu
Gormsen Erik
Halkier Torben
Ikegami Naoko
Takagi Shinobu
Fries Kery
Gregg, Esq. Valeta
Novonordisk A/S
Zelson Esq. Steve T.
LandOfFree
Enzymatic detergent composition does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Enzymatic detergent composition, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzymatic detergent composition will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-878671