Enzymatic decomposition method of chitin-containing materials

Details Enzymatic decomposition method of chitin-containing materials Enzymatic decomposition method of chitin-containing materials

1992-05-22

1993-11-16

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 72, 435 84, 435101, 435200, 435206, 435209, 536 20, C12P 1928, C12P 1926, C12N 936, C12N 942

Patent

active

052623106

ABSTRACT:
Chitin-containing material is heat-treated in organic solvent or in the solvent with water, and then .beta.-1, 4 glycoside decomposing enzyme is added for decomposing the chitin-containing material by enzyme reaction. Or, chitin-containing material is ultrasonicated in organic solvent or in the solvent with water, and then chitin-containing material is decomposed by enzyme reaction of .beta.-1, 4 glycoside decomposing enzyme. Further, chitin-containing material is exposed in the solution containing urea and/or surfactant, and then chitin-containing material is let co-exist with .beta.-1, 4 glycoside decomposing enzyme under the presence or non-presence of urea and/or surfactant for decomposing chitin-containing material. Or, at the time of exposing chitin-containing material in the solution containing urea and/or surfactant, the solution is heated and the .beta.-1, 4 glycoside decomposing enzyme is added for decomposing chitin-containing material.

REFERENCES:
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patent: 4536207 (1985-08-01), McCandliss
patent: 4686185 (1987-08-01), Wakunaga
Douzou et al "J. Mol. Biol." (1974) 96, 367-380.
Sharon "Proc Roy Soc" (ser B) 167, 402-415.
Blake et al "Proc Roy Soc" (ser B) 167, 378-388.

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