Enzymatic coupling reaction of N-protected-L-aspartic acid and p

Details Enzymatic coupling reaction of N-protected-L-aspartic acid and p Enzymatic coupling reaction of N-protected-L-aspartic acid and p

1996-10-11

1997-12-02

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435212, 435213, 435219, 530333, 560 38, 560 40, 560 41, C12P 2100, C12P 2102, C07K 506

Patent

active

056934858

ABSTRACT:
The invention relates to a process for the preparation of N-protected-L-aspartyl-L-phenylalanine methyl ester by enzymatic coupling of an N-protected-L-aspartic acid and L- or DL-phenylalanine methyl ester in an aqueous solution with formation of a precipitate using a thermolysin-like protease enzyme, wherein a water-immiscible organic solvent is added to and blended with the reaction system during the formation of the precipitate in the course of the coupling reaction, which organic solvent has a relatively high affinity for the precipitate. The process is particularly suitable as a continuous process. The method results in lower enzyme deactivation during and after the enzymatic coupling reaction and in larger and thicker precipitated crystals.

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Database WPI, Section Ch, Week 8531, Derwent Publications Ltd., London, GB, Class A96, JP-A-60-118190 (Jun. 1995), AN 85-188004, Abstract.

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