Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1994-12-29
1996-06-18
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435219, 435220, 435221, 435222, 560 38, 560 41, 560 49, C12P 2102, C12N 950, C12N 952, C12N 954
Patent
active
055276890
ABSTRACT:
The invention relates to a process for the preparation of N-benzyloxycarbonyl-.alpha.-L-aspartyl-L-phenylalanine methyl ester by enzymatic coupling of N-benzyloxycarbonyl-L-aspartic acid and L-phenylalanine methyl ester in an aqueous medium with formation of a precipitate, the coupling reaction being effected with (virtually) equimolar quantities of N-benzyloxycarbonyl-L-aspartic acid and L-phenylalanine methyl ester under the influence of a neutral protease at an initial pH of from 4.5 to 6.0 and in the presence of from 3 to 25%, calculated as per cent by weight based on the total reaction mixture, of an alkali metal salt, alkaline earth metal salt or ammonium salt.
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Chemical Abstracts, vol. 120, No. 35, Abstract No. 48817v 31 Jan. 1994.
Journal of the Chemical Society, Perkin Transactions II, 356-360 (1981).
Irino Shigeaki
Nakamura Shin-ichiro
Oyama Kiyotaka
Quaedflieg Peter J. L. M.
Van Dooren Theodorus J. G. M.
Holland Sweetener Company V.O.F.
Lilling Herbert J.
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