Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Treating animal or plant material or micro-organism
Patent
1983-08-01
1986-09-02
Marantz, Sidney
Chemistry: molecular biology and microbiology
Process of utilizing an enzyme or micro-organism to destroy...
Treating animal or plant material or micro-organism
435 2, 435200, 435208, 424 88, 424101, C07K 300, A01N 102, C12N 924, C12N 940
Patent
active
046096272
ABSTRACT:
Type O erythrocytes are produced from certain subtypes of A erythrocytes or type AB erythrocytes by contacting the same following equilibration of a pH of 5.6-5.8 with an .alpha.-N-acetylgalactosaminidase, preferably obtained from an avian liver, for periods sufficient to convert the A antigen in the erythrocyte to the H antigen. Following removal of the enzyme, the erythrocyte is re-equilibrated to a pH of 7.2-7.4. As a result, there is obtained O type erythrocytes characterized by a 60 to 90 percent ATP level based on the level of ATP in naturally occurring O or AB erythrocytes. Beginning with certain A cells one obtains synthetic O erythrocytes characterized by a terminal .alpha.-fucose moiety, O antigenicity, and the absence of A antigenicity. Beginning with A.sub.2 B erythrocytes, one obtains B erythrocytes by the same process characterized by the absence of A antigenicity, greater H antigenicity than naturally occurring A.sub.2 B cells, the presence of B antigenicity and the aforedescribed ATP levels.
REFERENCES:
patent: 4112070 (1978-09-01), Harmening
patent: 4330619 (1982-05-01), Goldstein
patent: 4427777 (1984-01-01), Goldstein
Levy, G. N. et al., Journal of Biol. Chem., vol. 255, (24), 11737-11742 (1980).
Goldstein, J. et al., Science, vol. 215, (4529) (pp. 168-170), Jan. 8, 1982, (Goldstein, III).
Uda, Yutaka et al., The Journal of Biological Chemistry, vol. 252, (15) pp. 5194-5200, (Aug. 10, 1977).
Krawczewicz L.
Marantz Sidney
New York Blood Center Inc.
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