Enzymatic cleaning compositions

Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – For cleaning a specific substrate or removing a specific...

Reexamination Certificate

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C510S138000, C510S119000, C510S238000, C510S239000, C510S226000, C510S235000, C510S305000, C510S320000, C510S321000, C510S323000, C510S392000, C510S530000

Reexamination Certificate

active

06440911

ABSTRACT:

FIELD OF THE INVENTION
The present invention relates to cleaning compositions comprising a mannanase and a carbohydrase selected from cellulases, amylases, pectin degrading enzymes and/or xyloglucanases.
BACKGROUND OF THE INVENTION
The use in detergents of carbohydrases is well-known in the art, for example, amylase enzymes have long been recognised in detergent compositions to provide the removal of starchy food residues or starchy films from dishware or hard surfaces or to provide cleaning performance on starchy soils as well as other soils typically encountered in laundry applications.
Additionally, the use of cellulase or related xyloglucanase is also well-known in the art. This activity in particular on fabrics provides a cleaning, rejuvenation, softening and generally improved handfeel characteristics to the fabric structure. The activity of cellulase is one in which cellulosic fibres or substrates are attacked by the cellulase and is depending on the particular function of the cellulase, which can be endo- or exo- cellulase and the respective hemicellulases. The cellulose structures are depolymerized or cleaved into smaller and thereby more soluble or dispersible fractions.
Pectin degrading enzymes are known to provide soil/stain removal benefits when used in washing and cleaning operations, specifically to provide the removal of a broad range of plant and fruit based stains and enhance the realistic item cleaning profile of the detergent compositions. Indeed, removal of stains stemming from plants, wood, mould-clay based soil and fruits is one of today's toughest cleaning task; in particular with the trends to move to low wash temperatures. Food soils are often difficult to remove effectively from a soiled substrate. Highly colored or “dried-on” soils derived from fruit and/or vegetable juices are particularly challenging to remove. Specific examples of such soils would include orange juice, tomato juice, banana, mango or broccoli soils.
However, the above described carbohydrases have an activity specific for their given substrates and their enzymatic activity may be limited on other types of substrates and/or complex substrates (Industrial Enzymology, Chap 2.13, Second Edition, T. Godfrey ISBN0-333-59464-9).
Food and cosmetic stains/soils represent the majority of consumer relevant stains/soils and often comprise food additives such as thickener/stabiliser agents. Indeed, hydrocolloids gums and emulsifiers are commonly used food additives. The term “gum” denotes a group of industrially useful polysaccharides (long chain polymer) or their derivatives that hydrate in hot or cold water to from viscous solutions, dispersions or gels. Gums are classified as natural and modified. Natural gums include seaweed extracts, plant extrudates, gums from seed or root, and gums obtained by microbial fermentation. Modified (semisynthetic) gums include cellulose and starch derivatives and certain synthetic gums such as low methoxyl pectin, propylene glycol alginate, and carboxymethyl and hydropropyl guar gum (Gums in
Encyclopedia Chemical Technology
4
th
Ed. Vol. 12, pp842-862, J. Baird, Kelco division of Merck). See also Carbohydrate Chemistry for Food Scientists (Eagan Press—1997) by R. L. Whistler and J. N. BeMiller, Chap 4, pp63-89 and Direct Food Additives in Fruit Processing by P. Laslo, Bioprinciples and Applications, Vol1, Chapter II, pp313-325 (1996) Technomie publishing. Some of these gums such as guar gum (E412), locust bean (E410) are widely used alone or in combinations in many food applications (Gums in ECT 4
th
Ed., Vol. 12 pp842-862, J. Baird, Kelco division of Merck).
The guar gum used in these food and cosmetic stains is obtained from the seed endosperm of the leguminous plant
Cyamopsis tetragonoloba
. The guar gum (also called guaran) extracted from the dicotyledonous seed is composed of a 1-4, b-D-mannopyranosyl unit backbone and is used as a thickening agent in dressing and frozen products and cosmetics (H.-D. Belitz, Food Chemistry pp 243, English version of the second edition, Springer-verlag, 1987, ISBN 0-387-15043-9(US)) & (Carbohydrate Chemistry for Food Scientists, R. L. Wilstler, eagan press, 1997, ISBN 0-913250-92-9) & (Industrial Gum, second editions, R. L. Whistler pp 308, Academic Press, 1973, ISBN, 0-12-74-6252-x). The locus bean gum (also called carob bean gum or St Jon's bread) is also used in the food industry and is extracted from the seed of an evergreen cultivated in the Mediterranean area. The locus bean gum probably differs from the structure of guar gum only in smaller number of D-galactosyl side chains and have the same 1-4, b-D-mannopyranosyl backbone. In leguminous seeds, water-soluble galactomanann is the main storage carbohydrate, comprising up to 20% of the total dry weight in some cases. Galactomannan has a &agr;-galactose linked to O-6 of mannose residues and it can also be acetylated to various degree on O-2 and O-3 of the mannose residues.
As seen from the above, there is a continuous need to formulate cleaning compositions which provide superior cleaning performance. This objective has been met by formulating cleaning compositions comprising a mannanase and a carbohydrase selected from cellulases, amylases, pectin degrading enzymes and/or xyloglucanases.
It has been surprisingly found that the combined use of a mannanase and one or more of the selected carbohydrases, provides superior cleaning due to the synergistic effect of the mixed enzyme system, i.e. superior stain removal, dingy cleaning and whiteness maintenance. Specifically, it has been found that the combined use of a mannanase and one or more of the selected carbohydrases provides outstanding stain removal on key stains, even at very low wash temperature and/or low detergent level.
It has been further found that the performance of the cleaning compositions of the present invention is enhanced by the addition of selected surfactants, a builder and/or a bleach system.
Mannanases have been identified in several Bacillus organisms. For example, Talbot et al., Appl. Environ. Microbiol., vol. 56, No. 11, pp. 3505-3510 (1990) describes a &bgr;-mannanase derived from
Bacillus stearothermophilus
in dimer form having a MW of 162 kDa and an optimum pH of 5.5-7.5. Mendoza et al., World J. Micobio. Boitech., vol. 10, no. 5, pp. 551-555 (1994) describes a &bgr;-mannanase derived from
Bacillus subtilisis
having a MW of 38 kDa, an optimum activity at pH 5.0/55° C. and a pl of 4.8. J0304706 discloses a &bgr;-mannanase derived from Bacillus sp. having a MW of 37+/−3 kDa measured by gel filtration, an optimum pH of 8-10 and a pl of 5.3-5.4. J63056289 describes the production of an alkaline, thermostable &bgr;-mannase, which hydrolyses &Dgr;-1,4-D-mannopyranoside bonds of e.g. mannans and produces manno:oligo:saccharides. J63036774 relates to a Bacillus micro-organism FERM P-8856 which produces &bgr; mannanase and &bgr;-mannosidase, at an alkaline pH. A purified mannanase from
Bacillus amyloliquefaciens
and its method of preparation useful in the bleaching of pulp and paper, is disclosed in WO97/11164. WO91/18974 describes an hemicellulase such as a glucanase, xylanase or mannanase, active at extreme pH and temperature and the production thereof. WO94125576 describes an enzyme exhibiting a mannanase activity derived from
Aspergillus aculeatus
CBS 101.43, that might be used for various purposes for which degradation or modification of plant or algae cell wall material is desired. WO93/24622 discloses a mannanase isolated from
Trichoderma reesie
for bleaching lignocellulosic pulps.
Amylase and cellulase enzymes are commonly used as detergent enzymes. Pectin degrading enzymes as detergent enzymes are described in EP-A-751 990 and in co-pending patent applications PCT/US96/12963, PCT/US96/12962, PCT/US96/12959, PCT/US96/12960 and PCT/US96/12691; all filed on Aug. 09, 1996. WO95/35362 discloses cleaning compositions containing plant cell wall degrading enzymes having a pectinase and/or hemicellulase and optionally cellulase activity for the removal of stains fr

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