Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1990-08-29
1993-03-23
Rollins, John W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435970, 422 52, 422 56, 422 57, 422 61, 422 8265, 422243, C12Q 100, G01N 2176, G01N 2100
Patent
active
051963027
ABSTRACT:
An apparatus for conducting a highly sensitive assay is provided wherein the level of a specific material is detected using a reaction which produces a detectable substance such as a colored dye or a fluorescent material in proportion to the concentration of the material being detected and which is carried out on an absorbent detecting layer comprising a superabsorbent polymeric material. The use of the superabsorbent polymeric materials greatly increases the sensitivity of the assay in a manner not achieved in the prior art because these polymeric materials are an excellent medium for the detection of the color changes caused by the reactants in the assays. These superabsorbent polymers offer increased sensitivity of up to about ten times greater than that which was possible using conventional backing materials as the absorbent layer in assays. The present invention comprises a quick, inexpensive, and accurate means for testing materials such as alcohol with a sensitivity that approaches 0.001% (w/v). A method of conducting colorimetric or other assays in accordance with the present invention is also provided. The present invention is especially useful in performing enzymatic assays.
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Edelberg Barry A.
McDonnell Thomas E.
Rollins John W.
The United States of America as represented by the Sectetary of
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