Enzymatic assays for glucose, creatine phosphokinase or plasma a

Details Enzymatic assays for glucose, creatine phosphokinase or plasma a Enzymatic assays for glucose, creatine phosphokinase or plasma a

1974-04-29

1976-05-11

Naff, David M.

Textiles: fiber preparation

195 63, 195 99, 1951035C, G01N 3114, C07G 702

Patent

active

039560694

ABSTRACT:
Enzymatic assays for determining glucose, creatine phosphokinase or plasma ammonia, wherein nicotinamide adenine dinucleotide is reduced or the reduced form of nicotinamide adenine dinucleotide is oxidized to nicotinamide adenine dinucleotide, are carried out in the presence of about 2 to 50 micromoles per liter of oxalic acid or oxamic acid, or salts thereof. The oxalic acid, oxamic acid and salts thereof inhibit lactic dehydrogenase which causes errors in the assays.

REFERENCES:
patent: 3388044 (1968-06-01), Babson
patent: 3540984 (1970-11-01), Deutsch
patent: 3764478 (1973-10-01), Bergmeyer et al.
patent: 3778350 (1973-12-01), Bergmeyer et al.
Rosalki, S. B., An Improved Procedure for Serum Creatine Phosphokinase Determination, J. Lab. & Chem. Med., Vol. 69, No. 4, 1967, (pp. 696-705).
Novoa et al., Lactic Dehydrogenase, J. Biol. Chem., Vol. 234, No. 5, 1959, (pp. 1143-1148).

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