Enzymatic assay method

Details Enzymatic assay method Enzymatic assay method

1983-10-03

1987-07-21

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 435 14, 435 19, 435 21, 435 22, 435 23, 435 24, 435 25, 435817, C12Q 100, C12Q 134, C12Q 144, G01N 3350

Patent

active

046818413

ABSTRACT:
A method for assaying a compound of the formula ##STR1## wherein R.sub.1 is hydroxyl or amino, or hydrogen if at least one of R.sub.2, R.sub.3, R.sub.4, R.sub.5 and R.sub.6 is hydroxyl or amino, and R.sub.2, R.sub.3, R.sub.4, R.sub.5 and R.sub.6 are hydrogen, halogen, lower alkyl, lower alkoxy, amino, substituted amino, hydroxy, carboxyl or sulfo, or R.sub.5 and R.sub.6 together form a ring, comprises establishing a reaction system containing the compound to be assayed and a coupler and an enzyme capable of consuming oxygen and generating a pigment in the presence of the compound and the coupler. A detectable change in the reaction system is measured, to assay the compound in question. The measurement can comprise the measurement of consumed oxygen, as by an oxygen electrode. Or the measurement can comprise the measurement of the generated pigment, as by a colorimetric assay at a specific absorption wavelength thereof.

REFERENCES:
patent: 4177109 (1979-12-01), Tohyama et al.
patent: 4209459 (1980-06-01), Nagasawa et al.
patent: 4384041 (1983-05-01), Matsumoto et al.
patent: 4472499 (1984-09-01), McCroskey
patent: 4529709 (1985-07-01), Takabayashi et al.

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