Enzymatic assay for homocysteine and a kit therefor

Details Enzymatic assay for homocysteine and a kit therefor Enzymatic assay for homocysteine and a kit therefor

1995-05-16

1997-05-20

Kight, John

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 18, 435 15, 435 21, 435810, 435975, 514499, C12Q 100, C12Q 134, G01N 3353, A01N 5502

Patent

active

056311273

ABSTRACT:
The invention relates to a method for assaying homocysteine in a sample such as blood, plasma or urine, which comprises the steps of contacting the sample with a homocysteine converting enzyme and at least one substrate for the enzyme other than homocysteine, and without chromatographic separation, assessing a non-labelled analyte selected from a homocysteine co-substrate and the homocysteine conversion products of the enzymic conversion of homocysteine by said enzyme.

REFERENCES:
patent: 5124351 (1992-06-01), Rabinovitz et al.
patent: 5385933 (1995-01-01), Rabinovitz et al.
Bergmeyer, Methods of Enzymatic Analysis, 1985, pp. 110-117; 357-364; and 403-409.
Refsum et al., Clinical Chemistry, vol. 31, No. 4, Apr. 1985, pp. 624-628.
Ueland et al, Clin. Chem vol. 39(9), pp. 1764-1779 (1993).
Andersson et al, Clin. Chem. vol. 39(8), pp. 1590-1597 (1993).

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