Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1980-06-30
1982-01-05
Hruskoci, Peter A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 19, 435 26, 435198, 435810, 435884, C12Q 148, C12Q 144
Patent
active
043095020
ABSTRACT:
An assay which is capable of determining the endogenous glycerol and endogenous triglycerides content of a sample without the necessity of performing a separate glycerol assay. The assay of the instant invention can be performed in a single cuvette and is made possible by the use of a novel reagent kit.
The reagent kit is of the type comprising lipase; glycerol kinase; adenosine triphosphate; and at least one reagent capable of being used to assay glycerol-1-phosphate or adenosine diphosphate. This reagent kit is characterized in that the lipase employed therein is from Staphylococcus epidermidis NRRL B-12072. In the absence of an auxiliary agent, this lipase does not cleave triglycerides to glycerol. Accordingly, the reagent kit further comprises, as the auxiliary agent, a surfactant which is capable of activating the lipase. Because these surfactants do not absorb at either 340 or 520 nm, one can determine a sample's endogenous glycerol content and endogenous triglycerides content in a single cuvette.
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Beckman Instruments Inc.
Frieman Robert S.
Hruskoci Peter A.
Steinmeyer R. J.
Vanderburgh J. E.
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