Enzymatic antioxidant of allene oxide for lipid peroxidation in

Details Enzymatic antioxidant of allene oxide for lipid peroxidation in Enzymatic antioxidant of allene oxide for lipid peroxidation in

1997-07-17

2000-10-17

McElwain, Elizabeth F.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

514 2, 536 232, 530370, A61K 3843, C11B 900, C08B 300, C08B 500

Patent

active

061327115

ABSTRACT:
The present invention relates to the isolation and use of an allene oxide synthase enzyme as an antioxidant of lipid peroxides in biological systems. It is based, at least in part, on the discovery that antioxidation is accomplished enzymatically by RPP, a species of allene oxide synthase, in guayule, and on the discovery that the allene oxide synthase RPP disrupts the chain reaction and propagation steps of lipid peroxidation. The present further invention relates to the use of an allene oxide synthase to result in a time-dependent disappearance of conjugated dienes (i.e. lipid hydroperoxides). The allene oxide synthase rapidly converts free or esterified fatty acid peroxides or hydroperoxides into their corresponding epoxides, which, in turn are converted to ketols. The lipid peroxide and hydroperoxide substrates for this enzyme are known to be toxic to biological organisms and can generate additional peroxides by chain propagation reactions. In the presence of an allene oxide synthase these compounds are rapidly and effectively converted to allene oxides (the epoxide), thus breaking the chain reaction.

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