Environmentally safe parasitology fixative and stain

Details Environmentally safe parasitology fixative and stain Environmentally safe parasitology fixative and stain

1994-06-01

1996-04-16

Czaja, Donald E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving fixed or stabilized, nonliving microorganism,...

435 4, 435 29, 435 4051, C12Q 108, C12Q 300, C12Q 102

Patent

active

055081754

ABSTRACT:
A fixative-stain system, which gives superior preservation of nuclear detail, is free from toxic mercury compounds, and which is simple and easy to use, includes a zinc salt and a cobalt salt, in combination, as a fixative, and at least one of Chlorazol Black E, Fast Green FCF and May-Grunwald stains, and preferably the three in admixture, as a staining composition. The fixative may also be used alone. The present fixative-stain system is suitable for fixing and staining all types of parasites such as enteric and other parasites which infect animals and humans.

REFERENCES:
patent: 4666699 (1987-05-01), Slifkin
patent: 5260048 (1993-11-01), Ryan
Walter B. Wheatley, A Rapid Staining Procedure For Intestinal Amoebae and Flagellates, American Journal of Clinical Pathology, vol. 21 (Oct. 1951), pp. 990-991.
Lynne S. Garcia et al., Diagnostic Medical Parasitology, Elsevier Science Publishing Co., (1988), pp. 369-391.
Lynne S. Garcia et al., Evaluation of Intestinal Protozoan Morphology in Polyvinyl Alcohol Preservative: . . . , Journal of Clinical Microbiology, vol. 31, No. 2 (Feb. 1993), pp. 307-310.
Peter M. Banks, Technical Aspects of Specimen Preparation and Special Studies, Surgical Pathology of the Lymph Nodes and Related Organs, Ed. Jaffe, Saunders Publishing Co. (1985) pp. 1-21.
Lennette et al. Manual of Clinical Microbiology Third Edition ASM (1980) pp. 677-687, 1010-1011.
Papamiltiades Cancer Cytology vol. 13, No. 1 (no pages given) 1973.
The Merck Index Merck & Co Ninth Ed (1976) p. 1309.

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