Enucleation of avian eggs by gamma irradiation

Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal – Via microinjection of a nucleus into an embryo – egg cell – or...

Reexamination Certificate

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C435S447000

Reexamination Certificate

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10498286

ABSTRACT:
A method of enucleating an egg by exposing the egg to gamma irradiation. The dose of gamma irradiation used is sufficiently high to halt development of the egg or embryo derived therefrom directed by the nucleus of the irradiated egg, but sufficiently low to enable development of the egg or embryo directed by a nucleus transferred into the enucleated egg.

REFERENCES:
patent: 2006/0010510 (2006-01-01), Christmann
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Nour, M.S. Mohamed et al., “Preparation of Young Preactivated Oocytes with High Enucleation Efficiency for Bovine Nuclear Transfer”,Theriogenology, 51:661-666 (1999).
Dominko, T. et al., “Dynamic Imaging of the Metaphase II Spindle and Maternal Chromosomes in Bovine Oocytes: Implications for Enucleation Efficiency Verification, Avoidance of Parthenogenesis, and Successful Embryogenesis”,Biology of Reproduction, 62:150-154 (2000).
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