Entomopathogenic nematode for control of insect pests

Drug – bio-affecting and body treating compositions – Preparations characterized by special physical form – Biocides; animal or insect repellents or attractants

Reexamination Certificate

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C424S093700, C424S408000, C424S451000, C424S458000, C424S489000

Reexamination Certificate

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07014859

ABSTRACT:
A nematode collected fromDiaprepes abbreviatuswas identified and described as a new species,Steinernema diaprepesin. sp. The new species can be distinguished from other species of the genus by the following characteristics: Males: Spicule averaging 79 (71–90) Fm; the ratio D % (distance from the anterior end/esophagus length×100) about 80; the ratio SW (spicule length/anal body width) about 1.8. Females: Vulva with short, double flapped epiptygma; tail terminus usually with 5 mucrons. Infective juveniles: Body averaging 1002 (880–1133) Fm, EP (distance from anterior end to excretory pore)=74 (66–83) Fm; tail length=83 (65–91) Fm, and E % (EP/tail length)=89.6 78–114). Lateral field pattern variable, the formula for the arrangement of ridges from head to tail is: 2, 6, 7, 8, 4, 2. The portion with 8 ridges is the longest. This new species can be differentiated from the 3 closest species,S. feltiae, S. glaseri, andS. oregonenseby characteristics of their sequences.

REFERENCES:
patent: 6184434 (2001-02-01), Raulston et al.
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Nguyen, Khuong B. et al., Scanning Electron Microscope Studies ofSteinernema glaseri(Nematoda: Steinernematidae), Nematologica, 1995, pp. 183-190, vol. 41.
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Nguyen, Khuong B. and Larry Duncan,Steinernema diaprepesin. sp. (Rhabditida: Steinernematidae), a Parasite of the Citrus Root WeevilDiaprepes abbreviatus(L) (Coleoptera: Curculionidae), Journal of Nematology, 2002, pp. 159-170, vol. 34, No. 2.
Poinar, George O. Jr., Taxonomy and Biology of Steinernematidae and Heterorhabditidae, Entomopathogenic Nematodes in Biological Control, 1990, pp. 23-61.

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