Enterokinase cleavage sequences

Details Enterokinase cleavage sequences Enterokinase cleavage sequences

2005-06-14

2005-06-14

Patterson, Jr., Charles L. (Department: 1652)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C435S219000

Reexamination Certificate

active

06906176

ABSTRACT:
Novel enterokinase cleavage sequences are provided. Also disclosed are methods for the rapid isolation of a protein of interest present in a fusion protein construct including a novel enterokinase cleavage sequence of the present invention and a ligand recognition sequence for capturing the fusion construct on a solid substrate. Preferred embodiments of the present invention show rates of cleavage up to thirty times that of the known enterokinase cleavage substrate (Asp)4-Lys-Ile.

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