Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2007-08-14
2007-08-14
Rao, Manjunath N. (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
C435S006120, C435S252330, C435S320100, C435S488000
Reexamination Certificate
active
10416364
ABSTRACT:
The invention relates to a process for the preparation of L-amino acids, especially L-lysine, L-valine, L-homoserine and L-threonine, by fermenting a microorganism of the genusEscherichiawhich has a mutation or deletion in the gene encoding aspartate ammonium lyase (aspA). This mutation results in the loss of aspA enzymatic activity in the microorganism
REFERENCES:
patent: 3647628 (1972-03-01), Nakayama et al.
patent: 4278765 (1981-07-01), Debabov et al.
patent: 4347318 (1982-08-01), Miwa et al.
patent: 6197590 (2001-03-01), Richaud et al.
patent: 7011961 (2006-03-01), Noh et al.
patent: 0 994 190 (2000-04-01), None
patent: 1 013 765 (2000-06-01), None
patent: WO99/18228 (1999-04-01), None
patent: WO 01/05939 (2001-01-01), None
patent: WO 01/92545 (2001-12-01), None
patent: WO 02/06459 (2002-01-01), None
patent: WO 03/008600 (2003-01-01), None
patent: WO 03/008602 (2003-01-01), None
patent: WO 03/008604 (2003-01-01), None
patent: WO 03/008616 (2003-01-01), None
Menkel et al. Influence of increased aspartate availability on lysine formation by a recombinant strain ofCorynebacterium glutamicumand utilization of fumarate, Appl Environ Microbiol. Mar. 1989; 55 (3): 684-8.
Alefounder, et al., “Cloning, Sequence Analysis and Over-expression of the gene for the Class II Fructose 1,6-Bisphosphate Aldolase ofEscherichia coli,” Biochem. J. 257:529-534 (1989).
Blattner, et al., “The Complete Genome Sequence ofEscherichia coliK-12,”Science 277:1453-1462 (1997).
Byrne, et al., “DNA Sequences of thecysKRegions ofSalmonella typhimuriumandEscherichia coliLinkage of thecysKRegions toptsH,” J. Bacteriol. 170:3150-3157 (1988).
Byrne, et al., “Nucleotide Sequence of theaceBGene Encoding Malate Synthase A inEscherichia coli,” Nucleic Acids Res. 16:9342-(1988).
Chandrasekhar, et al., “Purification and Properties of the groES Morphogenetic Protein ofEscherichia coli,” J. Biol. Chem. 261:12414-12419 (1986).
Chao, et al., “Selective Production of L-aspartic Acid and L-phenylalanine by Coupling Reactions of Aspartase and Aminotransferase inEscherichia coli,” Enzyme and Microbial Tech. 27:19-25 (2000).
Clarke, et al., “Nucleotide Sequence of thepntAandpntBGenes Encoding the Pyridine Nucleotide Transhydrogenase ofEscherichia coli,” Eur. J. Biochem. 158:647-653 (1986).
Cortay, et al., “Nucleotide Sequence and Expression of theaceKGene Coding for Isocitrate Dehydrogenase Kinase/Phosphatase inEscherichia coli,” J. Bacteriol. 170:89-97 (1988).
Erni, et al., “Glucose-Permease of the Bacterial Phosphotransferase System,”J. Biol. Chem. 261:16398-16403 (1986).
Falzone, et al., “L-Aspartase fromEscherichia coli: Substrate Specificity and Role of Divalent Metal Ions,”Biochem. 27:9089-9093 (1988).
Ferrante, et al., “Cloning of an Organic Solvent-Resistance Gene inEscherichia coli: The Unexpected Role of Alkylhydroperoxide Reductase,”Proc. Natl. Acad. Sci. USA 92:7617-7621 (1995).
Grabau, et al., “Nucleotide Sequence and Deduced Amino Acid Sequence ofEscherichia coliPyruvate Oxidase, a Lipid-Activated Flavoprotein,”Nucleic Acids Res. 14:5449-5461 (1986).
Hosono, et al., “Decreasing Accumulation of Acetate in a Rich Medium byEscherichia colion Introduction of Genes in a Multicopy Plasmid,”Biosci. Biotech. Biochem. 59:256-261 (1995).
Jahreis, et al., “Nucleotide Sequences of theilvH-fruRGene Region ofEscherichia coliK12 andSalmonella typhimuriumLT2,”Mol. Gen. Genet. 226:332-336 (1991).
Jayasekera, et al., “Evaluation of Functionally Important Amino Acids in L-Aspartate Ammonia-Lyase fromEscherichia coli,” Biochemistry 36:9145-9150 (1997).
Landgraf, et al., “The Role of H-NS in One Carbon Metabolism,”Biochimie 76:1063-1070 (1994).
Lu, et al., “Molecular Cloning and Characterization of thepgmGene Encoding Phosphoglucomutase ofEscherichia coli,” J. Bacteriol. 176:5847-5851 (1994).
Matsuoka, et al., “Isolation, Hyperexpression, and Sequencing of theaceAGene Encoding Isocitrate Lyase inEscherichia coli,” J. Bacteriol. 170:4528-4536 (1988).
McPherson, et al., “Complete Nucleotide Sequence of theEscherichia coli gdhAGene,”Nucleic Acids Res. 11:5257-5267 (1983).
Medina, et al., “Sequence of thepckAGene ofEscherichia coliK-12: Relevance to Genetic and Allosteric Regulation and Homology ofE. coliPhosphoenolpyruvate Carboxykinase with the Enzymes fromTrypanosoma bruceiandSaccharomyces cerevisiae,” J. Bacteriol. 172:7151-7156 (1990).
Menkel, et al., “Influence of Increased Aspartate Availability on Lysine Formation by a Recombinant Strain ofCorynebacterium glutamicumand Utilization of Fumarate,”Applied Environ. Microbiol. 55:684-688 (1989).
Niersbach, et al., “Cloning and Nucleotide Sequence of theEscherichia coliK-12ppsAGene, Encoding PEP Synthase,”Mol. Gen. Genet. 231:332-336 (1992).
Ostrowski, et al., “DNA Sequences of thecysBRegions ofSalmonella typhimuriumandEscherichia coli,” J. Biol. Chem. 262:5999-6005 (1987).
Ostrowski, et al., “Characterization of the Flavoprotein Moieties of NADPH-Sulfite Reductase fromSalmonella typhimuriumandEscherichia coli,” J. Biol. Chem. 264:15796-15808 (1989).
Overduin, et al., “Nucleotide Sequence of theugpGenes ofEscherichia coliK-12: Homology to the Maltose System,”Molec. Microbiol. 2:767-775 (1988).
Pon, et al., “Identification, Cloning, Nucleotide Sequence and Chromosomal Map Location ofhns, the Structural Gene forEscherichia coliDNA-Binding Protein H-NS,”Mol. Gen. Genet. 212:199-202 (1988).
Ravnikar, et al., “Structural and Functional Analysis of a Cloned Segment ofEscherichia coliDNA that Specifies Proteins of a C4Pathway of Serine Biosynthesis,”J. Bacteriol. 169:4716-4721 (1987).
Sabe, et al., “Molecular Cloning of the Phosphoenolpyruvate Carboxylase Gene,ppc, ofEscherichia coli,” Gene 31:279-283 (1984).
Saffen, et al., “Sugar Transport by the Bacterial Phosphotransferase System,”J. Biol. Chem. 262:16241-16253 (1987).
Takagi, et al., “Cloning and Nucleotide Sequence of the Aspartase Gene ofEscherichia coli,” Nucleic Acids Res. 13:2063-2075 (1985).
Valle, et al., “Nucleotide Sequence of the Promoter and Amino-Terminal Coding Region of the Glutamate Dehydrogenase Structural Gene ofEscherichia coli,” Gene 23:199-209 (1983).
Vipond, et al., “Defined Deletion Mutants Demonstrate that the Major Secreted Toxins Are Not Essential for the Virulence ofAeromonas salmonicida,” Infection and Immunity 66:1990-1998 (1998).
Vogel, et al., “Cloning and Sequence of themdhStructural Gene ofEscherichia coliCoding for the Malate Dehydrogenase,”Arch. Microbiol. 149:36-42 (1987).
Willins, et al., “Characterization of Lrp, anEscherichia coliRegulatory Protein That Mediates a Global Response to Leucine,”J. Biol. Chem. 266:10768-10774 (1991).
Woods, et al., “Structural and Functional Relationships Between Fumarase and Aspartase,”Biochem. J. 237:547-557 (1986).
Woods, et al., “Differential Roles of theEscherichia coliFumarases andfnr-dependent Expression of Fumarase B and Aspartase,”FEMS Microbiol. Letters 48:219-224 (1987).
English language abstract for WO 99/18228, reference B1 above.
English language abstract for WO 01/05939, reference B3 above.
Chowdhury Iqbal
Degussa - AG
Law Office of Michael A. Sanzo, LLC
Rao Manjunath N.
Sanzo Michael A.
LandOfFree
Enterobacteriaceae strains with an attenuated aspA gene for... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Enterobacteriaceae strains with an attenuated aspA gene for..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enterobacteriaceae strains with an attenuated aspA gene for... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3884593