Enrichment of phosphate peptides for proteomic analysis

Details Enrichment of phosphate peptides for proteomic analysis Enrichment of phosphate peptides for proteomic analysis

2007-04-24

2007-04-24

Tsang, Cecilia J. (Department: 1654)

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Separation or purification

Reexamination Certificate

active

10698106

ABSTRACT:
The present invention is a method, and a kit for carrying out the method, of separating phosphorylated peptides from a mixture of phosphorylated and unphosphorylated peptides. The method involves reacting a collection of peptides, in which some of the peptides have one or more phosphate groups, with a first resin. The peptides are then reacted with a capture ligand which reacts with the phosphate group of the phosphorylated peptides to form a bond. The capture ligand is used to separate the phosphorylated peptides from the unphosphorylated peptides.

REFERENCES:
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Fields et al. “Solid-phase peptide synthesis and solid-state NMR spectroscopy of [Ala-15N][Val]gramicidin A.” Proc. Natl. Acad. Sci. USA 1988, 85, 1384-1388.
Oda et al. “Enrichment analysis of phosphorylated proteins as a tool for probing the phosphoproteome.” Nat. Biotech. 2001, 19, 379-382.
Mercader et al. “Generation of anticalins with specificity for a nonsymmetric phthalic acid ester.” Anal. Biochem. 2002, 308, 269-277.
Kundu & Roy “Aminopropyl silica gel as a solid support for preparation of glycolipid immunoadsorbent and purification of antibodies.” J. Lipid Res. 1979, 20, 825-833.

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