Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1997-09-03
2000-03-21
Carlson, Karen Cochrane
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 681, 435 691, 435 697, 435 698, 4352351, 435239, 4353201, 436802, 530350, 5303871, 5303879, 536 241, C12Q 170, C12P 2106, C07K 1400, C07H 1700
Patent
active
060401362
ABSTRACT:
A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.
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Bass Steven
Garrard Lisa J.
Greene Ronald
Henner Dennis J.
Lowman Henry B.
Carlson Karen Cochrane
Genentech Inc.
Schwartz Timothy R.
Winter Daryl B.
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