Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...
Patent
1994-03-04
1998-02-10
Saucier, Sandra E.
Chemistry: molecular biology and microbiology
Maintaining blood or sperm in a physiologically active state...
436 17, 436 63, 435 724, 435372, 435377, A01N 102, G01N 3353, G12Q 104
Patent
active
057167761
ABSTRACT:
A method is presented for selective stimulation of fetal lymphocytes to divide in culture after treatment of mixed adult-fetal blood samples. This method is particularly useful for diagnosing genetic abnormalities of a fetus using fetal cells from a maternal blood sample. The method consists of preferential destruction of maternal lymphocyte response to low concentrations of mitotic stimulants which results in a relative increase in the number of responding fetal cells in mixed adult-fetal blood samples. In the preferred embodiment of the invention, blood samples from pregnant women are processed by the described methodology, in particular, the erythrocytes are removed from the maternal sample by centrifugation, filtration or preferential lysis with ammonium bicarbonate, the suspension is then subjected to an osmotic shock and exposed to mitotic stimulants in culture, and cells in mitosis are harvested for chromosome analysis. This test will enable detection of fetal chromosome abnormalities by analysis of maternal blood samples.
REFERENCES:
Merkatz, I.R. et al. (1984) "An association between low maternal serum .alpha.-fetoprotein and fetal chromosomal abnormalities" Am. J. Obstet. Gynecol. 148:886-894.
Bogart, M.H. et al. (1987) "Abnormal maternal serum chorionic gonadotropin levels in pregnancies with fetal chromosome abnormalities" Prenat. Diagn. 7:623-630.
Canick, J.A. et al. (1988) "Low second trimester maternal serum unconjugated oestriol in pregnancies with Down's syndrome" Br. J. Obstet. Gynecol. 95:330-333.
Selypes, A., R. Lorencz (1988) "A noninvasive method for determination of the sex and karyotype of the fetus from the maternal blood" Hum. Genet. 79:357-359.
Walknowska, J. et al. (1969) "Practical and theoretical implications of fetal/maternal lymphocyte transfer" The Lancet 1:1119-1122.
Boyer, S.H. et al. (1976) "Enrichment of fetal origin from adult-fetal blood mixtures via selective hemolysis of adult blood cells: an aid to antenatal diagnosis of hemoglobinopathies" Prenat. Diagn. 7:623-630.
Alter, B.P. et al. (1979) "Selective hemolysis of adult red blood cells: an aid to prenatal diagnosis of hemoglobinopathies" Blood 53(2):279-287.
Bogart Mark H.
Rosenbaum, Esq. David G.
Saucier Sandra E.
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