Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2008-06-10
2008-06-10
Tsang, Cecilia (Department: 1654)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S068100, C436S092000
Reexamination Certificate
active
07384754
ABSTRACT:
A method useful in the analysis of glycosylated proteins, in which a mixture containing glycosylated proteins and unglycosylated proteins is contacted with a resin that includes a nucleophile bound to a solid support via a linker. The contacting is performed under conditions sufficient to result in removal of the glycosyl group from the glycosylated proteins and to concomitantly result in the deglycosylated proteins covalently bound to the solid support. The deglycosylated proteins bound to the solid support may be rinsed to remove proteins that are not covalently bound to the solid support. The deglycosylated proteins are released from the solid support and may be subjected to further purification and/or analysis.
REFERENCES:
patent: 2004/0023306 (2004-02-01), Aebersold et al.
patent: 2005/0261475 (2005-11-01), Tseng et al.
patent: 2006/0141528 (2006-06-01), Aebersold et al.
Garlick et al. Characterization of Glycosylated Hemoglobins. Relevance to monitoring of diabetic control and anlysis of other proteins. J. Clin. Invest. 1983. vol. 71, pp. 1062-1072.
Klenk et al. Determination of Glycosylated Hemoglobin by Affinity Chromatography: Comparison with Colorimetric and Ion-Exchange Methods, and Effects of Common Interferences. Clin. Chem. 1982, vol. 28, No. 10, pp. 2088-2094.
Spiro. Mini Review. Protein glycosylation: nature, distribution, enzymatic formation, and disease implications of glycopeptide bonds. Glycobiology, 2002, vol. 12, No. 4, pp. 43R-56R.
Wells et al. Mapping Sites of 0-GlcNac Modification Using Affinity Tags for Serine and Thronine Post-translational Modifications. Molecular and Cellular Proteomics. Sep. 26, 2002. vol. 1, No. 10, pp. 791-804.
Lee et al. Applications of affinity chromatography in proteomics. Analytical Biochemistry. 2004. vol. 324, pp. 1-10.
Blitzer et al. A Purified Serum Glycopeptide from Controls and Cystic Fibrosis Patients. I. Comparison of their Mucociliary Activity on Rabbit Tracheal Explants. Pediatr. Res. 1982. vol. 16, pp. 203-208.
Blitzer et al. A Purified Serum Glycopeptide from Controls and Cystic Fibrosis Patients. III. The Association of the Ciliary Dyskinetic Activity with the Oligosaccharide Component. Pediatr. Res. 1984, vol. 18, pp. 540-543.
Agilent Technologie,s Inc.
Cordero Garcia Marcela M
Tsang Cecilia
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