Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Reexamination Certificate
2000-12-22
2008-12-23
Crouch, Deborah (Department: 1632)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
C435S366000, C435S377000, C435S378000
Reexamination Certificate
active
07468277
ABSTRACT:
The present invention relates to a method of separating multipotential neural progenitor cells from a mixed population of cell types. This method includes selecting a promoter which functions selectively in the neural progenitor cells, introducing a nucleic acid molecule encoding a fluorescent protein under control of said promoter into all cell types of the mixed population of cell types, allowing only the neural progenitor cells, but not other cell types, within the mixed population to express said fluorescent protein, identifying cells of the mixed population of cell types that are fluorescent, which are restricted to the neural progenitor cells, and separating the fluorescent cells from the mixed population of cell types, wherein the separated cells are restricted to the neural progenitor cells. The present invention also relates to an isolated human musashi promoter and an enriched preparation of isolated multipotential neural progenitor cells.
REFERENCES:
patent: 5750376 (1998-05-01), Weiss et al.
patent: 5753506 (1998-05-01), Johe
patent: 5851832 (1998-12-01), Weiss et al.
patent: 5874304 (1999-02-01), Zolotukhin et al.
patent: 5968829 (1999-10-01), Carpenter
patent: 6245564 (2001-06-01), Goldman et al.
patent: WO 96/38541 (1996-12-01), None
patent: WO 97/07200 (1997-02-01), None
patent: WO 98/32879 (1998-07-01), None
patent: WO 99/29279 (1999-06-01), None
patent: WO 99/49014 (1999-09-01), None
patent: WO 00/23571 (2000-04-01), None
Brustle et al. In vitro generated neural precursors participate in mammalian brain development. Proceed. Natl. Acad. Sci. Dec. 1997, vol. 94, pp. 14809-14814.
Azizi et al. Engraftment and migration of human bone marrow stromal cells implanted int he brains of albino rats—similarities to astrocyte grafts. Proceed. Natl. Acad. Sci. Mar. 1998, vol. 95, pp. 3908-3913.
Zimmerman, L. et al. Independent Regulatory Elements in the Nestin Gene Direct Transgene Expressin to Neural Stem Cells or Muscle Precursors. Neuron. Jan. 1994, vol. 12, pp. 11-24.
Goldman, S. Glia as Neural Progenitor Cells. Trends in Neuroscience. Nov. 2003, vol. 26, No. 11, pp. 590-596.
Keyoung et al. High-Yield Selection and Extraction of Two Promoter-Defined Phenotypes of Neural Stem Cells from the Fetal Human Brain. Nature Biotechnology. Sep. 2001, vol. 19, pp. 843-850.
Salim et al. Identification of Proteomic Changes During Differentiation of Adult Mouse Subventricular Zone Progenitor Cells. Stem Cells Dev. 2007, vol. 16, pp. 1-23.
Bonnert et al. Molecular Characterization of Adult Mouse Subventricular Zone Progenitor Cells During the Onset of Differentiation. Eur. J. Neurosci. 2006, vol. 24, No. 3, pp. 661-675.
Weiss et al, “Is There A Neural Stem Cell in the Mammalian Forebrain?,”Trends in Neuroscience, 19(9):387-393 (1996).
Goldman et al, “Neural Precursors and Neuronal Production in the Adult Mammalian Forebrain,”Annals of the New York Academy of Sciences, 835:30-55 (1997).
Wang et al, “Isolation of Neuronal Precursors by Sorting Embryonic Forebrain Transfected with GFP Regulated by the Tα1 Tubulin Promoter,”Nature Biotechnology, 16:196-201 (1998).
CytoTherapeutics, Inc., Press Release “CytoTherapeutics' Researchers First to Directly Isolate Normal Human Neural Stem Cells Key Step Toward Clinical Application of Proprietary Stem Cell Technology,”BW Healthwire(Nov. 2, 1999).
Gloster et al., “The Tα1 α-Tubulin Promoter Specifies Gene Expression as a Function of Neuronal Growth and Regeneration in Transgenic Mice,”The Journal of Neuroscience14(12): 7319-7330 (1994).
Okano et al., “Musashil, a Universal Marker for CNS Stem Cells of Vertebrate Animals,” Proceedings of the International Workshop, Tokyo, Japan (Feb. 25-26, 1999).
Zimmerman et al., “Independent Regulatory Elements in the Nestin Gene Direct Transgene Expression to Neural Stem Cells or Muscle Precursors,”Neuron12:11-24 (1994).
Roy et al., “Identification, Isolation, and Promoter-Defined Separation of Mitotic Oligodendrocyte Progenitor Cells From the Adult Human Subcortical White Matter,”The Journal of Neuroscience19:9986-9995 (1999).
Lothian et al., “An Evolutionarily Conserved Region in the Second Intron of the Human Nestin Gene Directs Gene Expression to CNS Progenitor Cells and to Early Neural Crest Cells,”Eur. Journal of Neuroscience9:452-462 (1997).
Sakakibara et al., “Mouse-Musashi-1, a Neural RNA-Binding Protein Highly Enriched in the Mammalian CNS Stem Cell,”Developmental Biology176:230-242 (1996).
Good et al., “The HumanMusashi Homolog 1(MSII) Gene Encoding the Homologue of Musashi/Nrp-1, a Neural RNA-Binding Protein Putatively Expressed in CNS Stem Cells and Neural Progenitor Cells,”Genomics52:382-384 (1998).
Okano, Annual Meeting of Japanese Society for Neuroscience (Jul. 7, 1999) (abstracts and slides).
Gage et al., “Survival and Differentiation of Adult Neuronal Progenitor Cells Transplanted to the Adult Brain,”Proc. Natl. Acad. Sci. USA92:11879-11883 (1995).
Yaworsky et al., “Heterogeneity of Neural Progenitor Cells Revealed by Enhancers in the Nestin Gene,” Developmental Biology 205:309-321 (1999).
Kawaguchi et al., “Concentration of Nerve Stem Cells Using Nestin-GFP Transgenic Mouse and Cell Sorter,” 22nd Annual Meeting of the Molecular Biology Society of Japan, Program and Presentation Abstract, p. 697 (2P-1064) (1999).
Sawamoto et al., “Analysis of Dopamine Nerve Cell [Dopaminergin Neuron] Differentiation Process Using GFP and FACS,” 22nd Annual Meeting of the Molecular Biology Society of Japan, Program and Presentation Abstract, p. 697 (2P-1067) (1999).
Goldman Steven A.
Okano Hideyuki
Cornell Research Foundation Inc.
Crouch Deborah
Japan Science and Technology Corporation
Nixon & Peabody LLP
LandOfFree
Enriched preparation of human fetal multipotential neural... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Enriched preparation of human fetal multipotential neural..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enriched preparation of human fetal multipotential neural... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4027981