Enone reductase and methods of making and using thereof

Details Enone reductase and methods of making and using thereof Enone reductase and methods of making and using thereof

2007-04-10

2007-04-10

Saucier, Sandra E. (Department: 1657)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

Reexamination Certificate

active

10505396

ABSTRACT:
An isolated enone reductase characterized by a molecular mass of 61300+/−5000 Da; NADPH and NADPH as co-factor; a temperature optimum of 55–60° C. at pH 7.4; a pH optimum of 4.5–8.5 and substrate specificity on ∝,β-unsatured ketones; a process for producing it from a microorganism and a process for producing levodione from ketoisophorone using such reductase.

REFERENCES:
patent: 1 074 630 (2001-02-01), None
Kataoka, M. et al., “Old Yellow Enzyme fromCandida macedoniensisCatalyzes the Stereospecific Reduction of the C═C Bond of Ketoisophorone,”Biosci. Biotechnol. Biochem.,vol. 66, No. 12, pp. 2651-2657 (2002).
Wanner, P. and Tressl, R., “Purification and Characterization of Two Enone Reductases fromSaccharomyces cerevisiae,” Eur. J. Biochem.,vol. 255, pp. 271-278 (1998).
Wada, M. et al., “Production of a Doubly Chiral Compound, (4R,6R)-4-Hydroxy-2,2,6-Trimethylcyclohexanone, by Two-Step Enzymatic Asymmetric Reduction,”Applied and Environmental Microbiology,vol. 69, No. 2, pp. 933-937 (2003).

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