Enhancer and silencer sequences isolated from the GPIIB promoter

Chemistry: molecular biology and microbiology – Vector – per se

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435 691, 4351721, 4351723, 4352401, 4352402, 4352523, 536 231, 536 232, 536 235, 536 241, C12N 1509, C12N 1563, C12N 1585, C07H 2104

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055653588

ABSTRACT:
An amplifier sequence and a silencer sequence of the GPIIb promoter are disclosed. The amplifier sequence includes sequence domains (I) and (II), and the silencer sequence includes sequence domain (III). The use of these sequences in genetic engineering is also described.

REFERENCES:
patent: 4740461 (1988-04-01), Kaufman
Journal of Biological Chemistry. vol. 267, No. 15, May 25, 1992, Baltimore, S, pp. 10370-10374; Prandini, M. H. et al.: "Characterization of a specific erythromegakaryocytic enhancer within the glycoprotein IIb promoter".
Biochemical and Biophysical Research Communications., vol. 156, No. 1, 14 Oct. 1988, Duluth, MN, US. pp. 595-601; Prandini, M. H., et al.: "Isolation of the human characterization of the 5' flanking region".
Biochemistry, vol. 29, No. 5, 1990, Easton, Pa US, pp. 1232-1244; Heidenreich, R. et al.: "Organization of the gene for platelet glycoprotein IIb".
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Uzan et al. (1991) Journal of Biological Chemistry, vol. 266, pp. 8932-8939.
Romero et al. (1990) Nature, vol. 344, pp. 447-449.

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