Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 25 or more amino acid residues in defined sequence
Patent
1999-03-12
2000-08-08
Low, Christopher S. F.
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
25 or more amino acid residues in defined sequence
435 721, 800 21, C07K 1452, G01N 35567, C12N 500
Patent
active
061003786
ABSTRACT:
A synthetic peptide with enhanced pro-fertility action was produced by inclusion of additional amino acids at the carboxyl end of a previously disclosed synthetic peptide. Improvement in bioactivity over the previously disclosed peptide was demonstrated. A direct comparison of an earlier known synthetic peptide and an extended peptide involved brief exposure of sperm in vitro to one or the other peptide at several concentrations. When sperm then were evaluated in vitro using an egg-membrane substrate, an increased percentage of sperm bound for cells exposed to the new extended peptide. Similarly, when fertility of sperm after artificial insemination was the criterion, a greater percentage of eggs was fertilized by sperm exposed to the new extended peptide. In one preferred embodiment, this enhanced pro-fertility action was achieved with a peptide having a 68 amino acid sequence (SEQ ID NO 1:): Cys-Gln-Ser-Leu-Gln-Glu-Tyr-Leu-Ala-Glu-Gln-Asn-Gln-Arg-Gln-Leu-Glu-Ser-As n-Lys-Ile-Pro-Glu-Val-Asp-Leu-Ala-Agr-Val-Val-Ala-Pro-Phe-Met-Ser-Asn-Ile-P ro-Leu-Leu-Leu-Tyr-Pro-Gln-Asp-Arg-Pro-Arg-Ser-Gln-Pro-Gln-Pro-Lys-Ala-Asn- Glu-Asp-Val-Cys-Val-Asn-His-His-His-His-His-His.
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Amann Rupert P.
Gill Surinder P. S.
Hammerstedt Roy H.
BioPore, Inc.
Low Christopher S. F.
Mohammed Abdel A.
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