Enhancement of ribozyme catalytic activity by A 2'-O-substituted

Details Enhancement of ribozyme catalytic activity by A 2'-O-substituted Enhancement of ribozyme catalytic activity by A 2'-O-substituted

1997-03-18

2000-07-11

Elliott, George C.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 435 911, 435 9131, 536 231, 536 232, 536 245, C12N 1511, C12Q 168

Patent

active

060874848

ABSTRACT:
Methods are disclosed for increasing ribozyme catalytic activity without reducing specificity, which methods comprise contacting an RNA molecule with a ribozyme and a 2'-O-substituted facilitator oligonucleotide. The present invention further provides compositions comprising a ribozyme and an effective amount of a 2'-O-methyl substituted facilitator oligonucleotide. The use of a facilitator, particularly a 2'-O-substituted facilitator, and more especially a 2'-O-methyl substituted facilitator, greatly enhances ribozyme catalytic activity, frequently making an otherwise inactive ribozyme active.

REFERENCES:
Sproat et al. NAR 18:41-49, 1990.
Goodchild, J., (1992) Nucleic Acid. Res., vol. 20, pp. 4607-4612.
Denman, RB., (1993) Nucleic Acid Res., vol. 21, pp. 4119-4125.
Nesbitt et al., (1994) Antisense Research and Development, vol. 4, pp. 243-249.
Jankowsky et al., (1996) Nucleic Acid Res., vol. 24, pp. 423-429.
Denman RB., (1996) FEBS. Lett., vol. 382, pp. 116-120.
Welch et al., (1996) Gene Therapy, vol. 3, pp. 994-1001.
Jankowsky et al., (1996) Biochemistry, vol. 35, pp. 15313-15321.
Perkins et al., (1996) Biochemistry, vol. 35, pp. 16370-16377.
Goodchild, J. (1997) Chapter 28. In: Ribozyme Protocols. (Ed: Turner, PC.) (Series Ed: Walker, JM. Methods In Molecular Biology, vol. 74) Human Press, Totowa, pp. 265-273.
Jankowsky et al., (1997) Nucleic Acid Res., vol. 25, pp. 2690-2693.
Uhlmann et al., (1990) Chemical Reviews 90:544-575.
Sarven et al., (1990). Science 247:1222.
Rossi et al., (1990) J. Cell. Biochem. Suppl. 14A, D428.
Muesing et al., (1985) Nature 313:450.
Haseloff et al (1988) Nature 334, 585-591.
Hortsch et al (1990) Develop. 110, 1327-1340.
McCormack et al (1990) Proced. Nat. Acad. Sci. 87, 5227-5231.
Burch et al (1991) J. Clin. Invest. 88, 1190-1196.
Hampel and Tritz, (1989) Biochem. 28:4929-4933.
Hampel et al., (1990) Nuc. Acids. Res. 18:299-304.
Fedor and Uhlenbeck, (1990) Proc. Natl. Acad. Sci. USA 87:1668-1672.
Koizumi et al., (1988) FEBS 239:285-288.
Lehman and Joyce, (1993) Nature 361:182-185.
Goodchild et al., (1988) Arch. Biochem. and Biophys. 263:401-409.
Uhlenbeck (1987) Nature 328:596-600.
Kutyavin, et al., (1988), FEBS 238:35-38.
Maher and Dolnick, (1988), Nucleic Acids Res. 16:3341-3358.
Goodchild and Kohli, (1991), Archives of Biochem. & Biophysics 284:386-391.
Sproat, et al., (1990), Nucleic Acids Res. 18:41-49.

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