Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1989-08-04
1992-12-22
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435189, 4351721, 4353201, 536 27, 935 10, 935 14, 530400, C12N 120, C12N 902, C12N 1500, C07K 1300
Patent
active
051734257
ABSTRACT:
This invention relates to a process for the enhancement of naphthalene dioxygenase activity in organisms that have been transformed with DNA encoding the expression of a multiple component naphthalene dioxygenase enzyme. Cells containing the naphthalene dioxygenase enzyme are capable of producing indigo when cultured in the presence of indole. The invention includes procedures to enhance naphthalene dioxygenase activity by transforming a host cell with a DNA sequence that encodes at least two ferredoxin polypeptides for each reductase polypeptide and iron-sulfur polypeptide, by growing transformed organisms in the presence of iron, or by transforming a host cell with a DNA sequence that encodes a ferredoxin analog.
REFERENCES:
patent: 4520103 (1985-05-01), Ensley, Jr.
Ensley et al., "Napthalene Dioxygenase: Purification and Properties . . . ", J. Bact. Aug. 1983, vol. 155, No. 2, pp. 505-511.
Kurkela et al., "Cloning, Nucleotide Sequence and Characterization of Genes Encoding Napthalene Dioxygenase . . . ", Gene, 73 (1988) 355-362.
Ensley, B. D., et al., "Expression of Naphthalene Oxidation Genes in Escherichia coli Results in the Biosynthesis of Indigo", Science, vol. 222, Oct. 14, 1983, pp. 167-169.
Ensley, B. D., et al., "Expression and Complementation of Napthalene Dioxygnase Activity in Escherichia coli", Microbial Metabolism and the Carbon Cycle: A symposium in honor of Stanley Dagley, Jul. 13-16, 1987, Hagedorn, et al., ed., pp. 437-455.
Ensley, B. D. and D. T. Gibson, "Napthalene Dioxygenase: Purification and Properties of a Terminal Oxygenase Component", Journal of Bacteriology, Aug. 1983, vol. 155, No. 2, pp. 505-511.
Ensley, B. D., et al., "Oxidation of Naphthalene by a Multicomponent Enzyme System from Pseudomonas sp. Strain NCIB 9816", Journal of Bacteriology, Mar. 1982, vol. 149, No. 3, pp. 948-954.
Yen, K. M. and I. C. Gunsalus, "Plasmid gene organization: Napthalene/salicylate oxidation", Proc. Natl. Acad. Sci. USA, Feb. 1982, vol. 79, pp. 874-878.
Ensley, Jr. Burt D.
Murdock Douglas C.
Serdar Cuneyt M.
Amgen Inc.
Hendricks Keith D.
Wax Robert A.
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