Enhancement of intracellular delivery and tissue targeting...

Drug – bio-affecting and body treating compositions – Conjugate or complex of monoclonal or polyclonal antibody,...

Reexamination Certificate

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C530S350000, C530S387100, C530S391700, C424S134100, C424S183100

Reexamination Certificate

active

10446422

ABSTRACT:
A method for enhancing intracellular delivery of effector molecules is provided. The method involves modifying selected antibodies with biotin and streptavidin, conjugating these antibodies with an effector molecule, and delivering the conjugated effector to an intracellular target specifically recognized by the antibody.

REFERENCES:
Muzykantov et al., “Streptavidin facilitates internalization and pulmonary targeting of an anti-endothelial cell antibody(platelet-endothelial cell adhesion molecule 1) :A strategy for vascular immunotargeting of drugs”,Proc. Natl. Acad. Sci. USA1999 96:2379-2384.
Muzykantov et al., “The Functional Effects of Biotinylation of Anti-angiotensin-Converting Enzyme Monoclonal Antibody in Terms of Targetingin Vivo”, Analytical Biochemistry1995 226:279-287.
Muzykantov et al., “Endothelial cells internalize monoclonal antibody to angiotensin-converting enzyme”,American Journal Physiology1996; L704-L713 vol. 270 (5pt.1).
Liu et al., “Constitutive and Antibody-induced Internalization of Prostate-specific Membrane Antigen1”,Cancer Research1998 58:4055-4060.
Kalofonos et al., “Imaging of Tumor in Patients with Indium-111-Labeled Biotin and Streptavidin-Conjugated Antibodies:Preliminary Communication”, Journal of Nuclear Medicine 1990 11:1791-1796.
Rosebrough S.F., “Two-step immunological approaches for imaging and therapy”, Quarterly Journal Nuclear Med. 1996 40:234-251 XP-002086486.

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