Enhancement of hybridization of nucleic acids by anionic polymer

Details Enhancement of hybridization of nucleic acids by anionic polymer Enhancement of hybridization of nucleic acids by anionic polymer

1984-11-19

1987-08-25

Keppinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 78, 435803, 435810, C12Q 168, C12N 1500

Patent

active

046892940

ABSTRACT:
The rate of hybridization between two complementary polynucleotide segments in an aqueous medium is increased by the presence of the anionic polymers polyacrylate and polymethacrylate. The acceleration effect is particularly useful in nucleic acid hybridization assays involving immobilization of sample nucleic acids and the use of labeled probes. Nonspecific binding of probe to nitrocellulose supports is substantially lower in the presence of the present polymers than in the presence of the prior art accelerator dextran sulfate. Polyacrylate is particularly advantageous since it has been found to be effective at low concentrations and is significantly less expensive than the prior art compound.

REFERENCES:
patent: 4302204 (1981-11-01), Wahl et al.
patent: 4358535 (1982-11-01), Falkow et al.
Chemical Abstracts, vol. 94, No. 8, 1981, p. 8, Abstract No. 47875v, Inaki, Y., et al. "Functional Monomers . . . Nucleic Acid Bases".
Chemical Abstracts, vol. 99, No. 20, 1983, p. 99, Abstract No. 160350m, Lion Corp., "Liquid Detergents".
Martinell, J. et al. Proc. Natl. Acad. Sci. USA, vol. 78, No. 8, Aug. 1981, pp. 5056-5060.
Subirana, J. A. et al. Biopolymers, vol. 44, 1966, pp. 171-187.

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