Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – For cleaning a specific substrate or removing a specific...
Patent
1995-05-22
1997-12-23
Lieberman, Paul
Cleaning compositions for solid surfaces, auxiliary compositions
Cleaning compositions or processes of preparing
For cleaning a specific substrate or removing a specific...
510392, 510530, 510374, 510375, 510376, 510378, 435192, 210632, C11D 3386, D06L 316
Patent
active
057007691
DESCRIPTION:
BRIEF SUMMARY
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a 371 of PCT/DK93/00393 filed Dec. 1, 1993, which is incorporated herein by reference.
TECHNICAL FIELD
This invention relates to activation of enzymes. More specifically, the invention relates to peroxidase enhancing agents.
The invention also relates to methods of oxidizing a substrate with a source of hydrogen peroxide in the presence of a peroxidase enzyme and a peroxidase enhancing agent. More specifically, the invention relates to a method of bleaching of dye in solutions, to a method of inhibiting the transfer of a textile dye from a dyed fabric to another fabric when said fabrics are washed together in a wash liquor, to a method of bleaching of lignin-containing material, in particular bleaching of pulp for paper production, to a method of treatment of waste water from pulp manufacturing, and to a method of enzymatic polymerization and/or modification of lignin or lignin containing material.
BACKGROUND ART
Peroxidases (E.C. 1.11.1.7) are enzymes that catalyse the oxidation of a substrate (an electron or hydrogen donor) with hydrogen peroxide. Such enzymes are known from microbial, plant and animal origins, e.g. peroxidase from Coprinus cinereus (cf. e.g. EP 179,486). They are typically hemoproteins, i.e. they contain a heme as a prosthetic group.
Use of peroxidase together with hydrogen peroxide or a hydrogen peroxide precursor has been suggested e.g. in bleaching of pulp for paper production, in treatment of waste water from pulp production, for improved bleaching in laundry detergents, for dye transfer inhibition during laundering, and for lignin modification, e.g. in particle board production.
The compound 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate), ABTS, supplied by Boehringer Mannheim, is a chromogenic substrate, and a common peroxidase and phenol oxidase assay agent. These enzymes catalyse the oxidation of ABTS by hydrogen peroxide and dioxygen, respectively, producing a greenish-blue colour, which process may be monitored photometrically.
ABTS has been found to form a stable radical cation when oxidized by a laccase enzyme (polyphenol oxidase, EC 1.10.3.2), and has been proposed to act as a redox mediator for oxidation of non-phenolic lignin model
Studies on demethylation and delignification of kraft pulp by a laccase enzyme in the presence of ABTS showed that the extent of partial demethylation by laccase was increased in the presence of ABTS 823-827!.
Certain oxidizable substrates, e.g. metal ions and phenolic compounds such as 7-hydroxycoumarin (7 HCm), vanillin (VAN), and p-hydroxybenzenesulfonate (pHBS), have been described as accelerators or enhancers, able to enhance bleaching reactions (cf. e.g. WO 92/18683, WO 92/18687, and Kato M. and Shimizu S., Plant Cell Physiol. 1985 26 (7), pp. 1291-1301 (cf. Table 1 in particular), or Saunders B. C., et al., Peroxidase, London, 1964, p. 141 ff).
This accelerator effect is thought to be ascribable to the formation of short-lifed radicals or other oxidised states of this substrate which participate in the bleaching or other modification of the coloured substance.
SUMMARY OF THE INVENTION
It is an object of the invention to provide an agent for enhancing the activity of peroxidase enzymes, and to provide a method of enhancing the activity of peroxidase enzymes. It has now surprisingly been found that the activity of peroxidases increases significantly in the presence of a chemical compound capable of generating a stable oxidized product which can act as an electron acceptor.
Accordingly, in its first aspect, the present invention provides an agent for enhancing the activity of a peroxidase enzyme, the agent being a substrate for the peroxidase enzyme, and capable of generating a stable electron acceptor when present in a concentration of up to 250 .mu.M.
In its second aspect, the invention provides a method of oxidizing a substrate with a peroxidase enzyme, in the presence of a source of hydrogen peroxide, and in the presence of a peroxidase enhancing agent of the inve
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Abstract of DD 147368.
Conrad Lars Sparre
Ebdrup S.o slashed.ren
Schneider Palle
Yde Birgitte
Fries Kery A.
Gregg, Esq. Valeta A.
Lieberman Paul
Novo Nordisk A S
Zelson Esq. Steve T.
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