Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1994-03-25
1996-04-30
Gitomer, Ralph J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 79, 435 791, 435968, 536 171, 540541, 544 69, 548110, C12Q 128, C07H 1500
Patent
active
055124518
ABSTRACT:
In enhanced chemiluminescent (ECL) reactions of a fused aromatic diacyl cyclic hydrazide such as luminol, a peroxidase enzyme catalyst, an oxidant such as hydrogen peroxide and an enhancer, it has been found advantageous to use a combination of an organoboron enhancer such as 4-biphenylboronic acid with a non boron-containing enhancer, especially a phenolic or aromatic amine enhancer, particularly 4-iodophenol. ECL reactions are useful in diagnostic assay.
REFERENCES:
patent: 5242842 (1993-09-01), Sundrehagen
Anspach F., High Performance Liquid Affinity . . . Anal Biochem 179:1 May 15, 1989, pp. 171-181.
British Technology Group Limited
Gitomer Ralph J.
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