Enhancement of antigen immunogenicity

Drug – bio-affecting and body treating compositions – Lymphokine

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424 8591, 424 88, 424 89, 424 92, 5303871, 5303881, 5303917, 530402, 530403, 530404, 530405, 530406, 53038875, 5303887, 435 71, 435 693, A61K 3900, C07K 300

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051942542

ABSTRACT:
A new method is described for eliciting IgG antibody response to proteins or synthetic peptides, particularly those that are weakly immunogenic, without the requirement for the use of adjuvants, thereby making it easier and safer to confer protection against pathogenic organisms. The antigen is coupled to a monoclonal antibody, specific for membrane determinants expressed on certain types of mammalian recipient cells, called antigen presenting cells. The monoclonal antibody acts as a "vector" or "delivery vehicle" for targeting foreign antigens onto such recipient cells. This targeting facilitates subsequent antigen recognition by helper T-cells, which are pivotal in helping the induction of antigen-specific IgG responses.

REFERENCES:
Carayanniotis et al, Adjuvant-Free IgG responses induced with antigen coupled to antibodies against Class II MHC; Nature, vol. 327 No. 6117, pp. 59-61, May, 1987.
Carayanniotis, et al, Delivery of Synthetic Peptides by Anti-Class II MHC Monoclonal Antibodies Induces Specific Adjuvant-Free IgG responses in vivo Molecular Immun. vol. 25, No. 9, pp. 907-911, 1988.
Barber et al, Antigen Delivery by Immunotargeting: A Possibility for Adjuvant-Free Vaccines? Technological Advances in Vaccine Development, pp. 471-481, 1988.
Experimentia, vol. 18, No. 12, Aug. 8, 1962; pp. 553-554, Basel, CH; L. Forro et al. "A New Type of Antigen Induced by Chemical Linkage of Mycobacterium tubercolosis and y-Globulin".
Proceedings of the National Academy of Sciences, vol. 82, Oct. 1985, pp. 6652-6656, U.S. G. Klein "Monoclonal Antibodies Against Human Ia Antigens Stimulate Monocytes Interleukin 1".

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