Enhanced yeast transcription employing hybrid GAPDH promoter reg

Details Enhanced yeast transcription employing hybrid GAPDH promoter reg Enhanced yeast transcription employing hybrid GAPDH promoter reg

1989-07-18

1992-02-18

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 692, 435 693, 435189, 435192, 4353201, 435255, 435256, 4351723, 935 37, 536 27, C12P 2100, C12P 700, C07H 1512

Patent

active

050893980

ABSTRACT:
Yeast promoters of glycolytic enzymes are modified by isolating a fragment encompassing the RNA polymerase binding site and joining to the 5' end of this fragment a DNA sequence providing for enhanced inducible or constitutive transcription of a structural gene. Constructs are prepared for efficient expression of foreign genes in yeast.
Yeast strains 2150-2-3 (pC1/1GAPSOD) and AB110 (pC1/1GAPATi9), producing human .alpha..sub.1 -antitrypsin and superoxide dismutase, were desposited at the A.T.C.C. on May 9, 1984 and given Accession Nos. 20708 and 20709, respectively; and 2150-2-3 (GAP5), 2150-2-3 (Pyk5) and 2150-2-3 (PHO5GAP1), expressing Hepatitis B surface antigen, were deposited at the A.T.C.C. on May 9, 1984 and given Accession Nos. 20705, 20706 and 20707, respectively.

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Dobson et al., (1982) Nucleic Acids Res. 10: 2625-37.
Holland et al., (1979) J. Biol. Chem. 254: 5466-74.

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