Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or...
Reexamination Certificate
2005-02-15
2005-02-15
McKelvey, Terry (Department: 1636)
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
C435S320100
Reexamination Certificate
active
06855534
ABSTRACT:
In the present invention, viruses, plasmids or both are constructed which contain viral DNA, at least one head-to-head ITR junction, and recombinase recognition sites positioned such that site-specific recombination between recombinase recognition sites in separate plasmids results in generation of infectious viral DNA at high-efficiency in cotransfected host cells that have been engineered to express a site-specific recombinase. Because of the high-efficiency and specificity of the Cre enzyme, suitably engineered plasmids can be readily recombined to produce infectious virus at high-efficiency in cotransfected 293 cells, without, at the same time, producing wild-type adenovirus, with the attendant problems for removal thereof. Use of recombinases besides Cre and recombinase recognition sites besides lox sites, and use of cells other than 293 cells are also disclosed and enabled, as are kits incorporating the site-specific vector system, as well as compositions and methods for using such compositions as vaccines or in gene therapeutic applications. Enhancement in the efficiency of site-specific recombination is provided by inclusion of a head-to-head ITR junction in each virus, plasmid, or other nucleic acid construct.
REFERENCES:
patent: 6379943 (2002-04-01), Graham et al.
patent: WO 9725446 (1997-06-01), None
Bett et al., Proc. Natl. Acad. Sci. USA 91:8802-8806 (1994).*
Bett et al., J. Virology, 67:5911-5921 (1993).
Graham Frank L.
Ng Philip
Parks Robin
Beusse Brownlee Wolter Mora & Maire P.A.
Fischer Joseph
McKelvey Terry
Merck & Co. , Inc.
Vogel Nancy T.
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