Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1989-12-18
1992-01-21
Teskin, Robin L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 614, 435 695, 435 698, 435 699, 435212, 435219, 435 693, 536 27, 935 48, 935 37, 935 47, C07H 1512, C12P 2100, C12N 116
Patent
active
050827839
ABSTRACT:
Improved secretion of heterologous proteins by hosts such as yeast by using promoters of at most intermediate strength with heterologous DNA secretion signal sequences is disclosed. A promoter of at most intermediate strength, such as the actin (ACT) or iso-1-cytochrome c (CYC1) promoter in S. cerevisiae is operatively linked to a DNA signal sequence, such as the Mf.alpha.1 signal sequence. A DNA sequence for a selected protein, such as somatomedin C (SMC), tissue plasminogen activator (TPA) or tumor necrosis factor (TNF), may be operatively linked to the DNA signal sequence.
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Ernst Joachim F.
Schmeissner Ursula
Biogen Inc.
Haley, Jr James F.
Pierri Margaret A.
Teskin Robin L.
Yankwich Leon R.
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