Enhanced recovery of transformed cells

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

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C435S173600, C435S461000

Reexamination Certificate

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06849455

ABSTRACT:
DNA is efficiently transformed into a host by electroporation in the presence of a methylation package, which greatly improves the efficiency of the transformation. The methylation package comprises a source of cysteine, such as cysteine, homocysteine, or glutathione, with optional iron and magnesium ions.

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patent: 6214622 (2001-04-01), Treco et al.
patent: 6214804 (2001-04-01), Felgner et al.
High Efficiency Buffer Kits Feb. 2, 2001, [online], [retrieved on Dec. 13, 2002] Retrieved from EquiBio website using Internet <URL:http:/www.equibio.com/optimix.htm>.

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