Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1992-04-16
1996-06-04
Jacobson, Dian C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
43525233, 43525421, 530418, 530420, C12N 115, C12N 121, C12N 1509, C07K 100
Patent
active
055232159
ABSTRACT:
A method for isolating a desired protein which comprises: providing a host cell expressing the desired protein as an insoluble aggregate; culturing the host cell with an effective mount of Cu.sup.++ ; disrupting the host cell producing a lysate; incubating the insoluble fraction non-disulfide-bond reducing or non-copper competing chaotropic conditions to solubilize contaminants; separating the insoluble from the soluble fraction; and exposing the insoluble fraction to disulfide-bond reducing or copper competing chaotropic conditions to solubilize the desired protein.
REFERENCES:
patent: 4656131 (1987-04-01), Kitano
patent: 4748234 (1988-05-01), Dovin
patent: 5342921 (1994-08-01), Cousens
Mitraki et al., Protein Folding Intermediates and Inclusion Body Formation (1989) Biotechnology 7: 690-697.
Georgiou, G., AIChE Journal, 34(8):1233-1248 (1988).
Cousens Lawrence S.
Tekamp-Olson Patricia
Blackburn Robert P.
Chiron Corporation
Chung Ling-Fong
Green Grant
Jacobson Dian C.
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