Enhanced protein production in higher plants by N-terminal...

Details Enhanced protein production in higher plants by N-terminal... Enhanced protein production in higher plants by N-terminal...

2006-08-08

2006-08-08

Fox, David T. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

Nonplant protein is expressed from the polynucleotide

C800S278000, C435S069700, C435S320100, C435S419000, C435S468000, C536S023400, C536S023720

Reexamination Certificate

active

07087811

ABSTRACT:
Methods are disclosed for enhancing protein production. One method comprises preparing a vector by inserting a gene encoding ubiquitin in front of a gene encoding a protein of interest and inserting the vector into a cell. A fusion protein will be expressed which includes ubiquitin plus the protein of interest. Ubiquitin C-terminal hydrolases can cleave the fusion protein leaving the desired protein in its free state. This method causes enhanced production of the protein of interest as compared to performing the same method without the ubiquitin gene as part of the vector. A ubiquitin promoter is unnecessary to yield this enhanced production and is not used. A second method is very similar except that in place of a ubiquitin gene, a gene encoding fourteen amino acids of cucumber mosaic virus coat protein is inserted in front of the gene of interest. This results in expression of a fusion protein comprising the fourteen amino acid residues of the coat protein bonded to the protein of interest. The fusion protein is produced at a higher level than is the protein when the coat protein gene fragment is not present in the vector. In both methods the genes can be placed under the control of heterologous promoters such as a 35S promoter.

REFERENCES:
patent: 5773705 (1998-06-01), Vierstra et al.
patent: 0 672 754 (1995-09-01), None
patent: WO 90/02189 (1990-03-01), None
patent: WO 96/03519 (1996-02-01), None
patent: WO 96/03522 (1996-02-01), None
patent: WO 96/21018 (1996-07-01), None
Ecker et al., J. Biol. Chemistry. 1989, vol. 264, No. 13, pp. 7715-7719.
Garbarino et al., “Isolation of a ubiquitin-ribosomal protein gene (ubi3) from potato and expression of its promoter in transgenic plants,” Plant Molecular Biology 24:119-127, 1994.
Garbarino et al., “Isolation of a Polyubiquitin Promoter and Its Expression in Transgenic Potato Plants,” Plant Physiol. 109:1371-1378, 1995.
Genschik et al., “Structure and promoter activity of a stress and developmentally regulated polyubiquitin-encoding gene ofNicotiana tabacum,” Gene 148:195-202, 1994.
Haq et al., “Biological, serological and molecular characterization of a cucumber mosaic virus isolate from India,” Plant Pathol. 45:823-828, 1996.
Karrer et al., “Cloning of tobacco genes that elicit the hypersensitive response,” Plant Mol. Biol. 36:681-690, 1998.
GenBank Accession No. X89652 (Mar. 25, 1997).
GenPept Accession No. AAC49970 (Mar. 18, 1998).

Affiliated with

Also associated with

Rating

Enhanced protein production in higher plants by N-terminal... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enhanced protein production in higher plants by N-terminal..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enhanced protein production in higher plants by N-terminal... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3666525