Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1994-08-25
1998-12-01
Elliot, George C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351721, 4352351, 435236, 4352523, 4352528, 4353201, 435375, 536 231, 536 2372, C12N 1500, C12N 700, C12N 120, C12P 2100
Patent
active
058437037
ABSTRACT:
Compositions for enhancing the production of toxic polypeptides in prokaryotic hosts comprise a coding nucleic acid and a bacteriophage nucleic acid. The coding nucleic acid encodes a promoter operably linked to a gene encoding a selected toxic protein. The bacteriophage nucleic acid contains at least one gene from the early region of a T7-like bacteriophage other than an RNA polymerase. When both the coding nucleic acid and the bacteriophage nucleic acid are used to transform or transfect a prokaryotic host, the production of the toxic polypeptide is greater than the level of production of the same toxic polypeptide when the same host is transformed only with the coding nucleic acid.
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Brown William C.
Campbell Judith L.
California Institute of Technology
Elliot George C.
M'Garry Sean
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