Enhanced production of recombinant proteins in myeloma cells

Details Enhanced production of recombinant proteins in myeloma cells Enhanced production of recombinant proteins in myeloma cells

1986-09-24

1989-12-26

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 4352402, 435320, 435 694, 435 695, 435 6951, 435 6952, 435 696, 935 23, 935 32, 935 33, 935 70, 935 89, 935106, C12P 2100, C12N 1500

Patent

active

048898023

ABSTRACT:
A mammalian myeloma cell comprising a double-stranded DNA molecule in its genome containing a coding sequence encoding a non-immunoglobulin protein, a non-immunoglobulin promoter sequence adjacent to the 5' terminus of said coding sequence, and the 8-base pair nucleotide sequence 5'-ATTTGCAT-3' located 5' to the transcription initiation site of said promoter sequence. The DNA molecule may optionally contain an enhancer element. Methods of producing non-immunoglobulin protein and DNA molecules are also provided.

REFERENCES:
patent: 4663281 (1987-05-01), Gillies et al.
Sen et al., Cell, vol. 46, 705-716, Aug. 1986.
Karin et al., Cell, vol. 36, 371-379, Feb. 1984.
Singh et al., Nature, vol. 319, 1986 pp. 154-158.

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