Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2007-10-02
2007-10-02
Mehta, Ashwin (Department: 1638)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S468000, C435S418000, C435S419000, C800S278000
Reexamination Certificate
active
10766389
ABSTRACT:
Adding at least one gene involved in plant host cell T-DNA integration enhances transformation byAgrobacterium. The histone H2A gene encoded by theArabidopsisRAT5 gene increases transformation frequencies of plants, most likely by causing overexpression of a product needed for T-DNA integration.Agrobacterium tumefaciensgenetically transforms plant cells by transferring a portion of the bacterial Ti-plasmid, designated the T-DNA, to the plant, and integrating the T-DNA into the plant genome. However, not all plants are transformable byAgrobacteriumand transformation frequencies may be too low to be useful. Little is known about the T-DNA integration process, and no plant genes involved in integration have been identified prior to the present invention.
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Gelvin Stanton B.
Mysore Kirankumar S.
Barnes & Thornburg LLP
Martin Alice O.
Purdue Research Foundation
Zheng Li
LandOfFree
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