Enhanced luminescent or luminometric assay

Details Enhanced luminescent or luminometric assay Enhanced luminescent or luminometric assay

1984-02-09

1986-07-01

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 7, 435 8, 435810, 436508, 436531, 436800, 436817, 544234, 544237, G01N 3353, G01N 33564, C12Q 166, C12Q 128

Patent

active

045980446

ABSTRACT:
A phenolic compound is used to enhance the sensitivity of a luminescent reaction such as carried out in an immunoassay between a peroxidase enzyme, an oxidant, and a chemiluminescent 2,3-dihydro-1,4-phthalazinedione. Preferably, the phenolic compound is 4-iodophenol, 4-phenylphenol or 2-chloro-4-phenylphenol. In the preferred embodiment, horseradish peroxidase is coupled to an antibody to the substance to be assayed.

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