Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1985-07-29
1988-03-08
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 7, 435810, 422 52, 436800, C12Q 128, G01N 5300, G01N 2152
Patent
active
047299509
ABSTRACT:
It has been a problem that the chemiluminescent emission produced by the peroxidase-catalyzed oxidation of a 2,3-dihydro-1,4-phthalazinedione is often weak or gives a poor signal-background ratio and therefore does not give the high degree of sensitivity required for assay purposes, particularly for assay of peroxidase used to label a reagent for example in ELISA. The present invention solves the problem by the finding the certain narrowly defined aromatic amines are enhancers of the reaction and accordingly provides for their use in an assay and kit. The amines are of general formula (I) ##STR1## wherein the R symbols (that is, R, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5 and R.sup.6) have any of the meanings (a) to (j) given below, all other R symbols in each meaning are hydrogen atoms and the fused rings are to be read in the same configurational sense as formula I:
REFERENCES:
patent: 4290773 (1981-09-01), Magers et al.
patent: 4598044 (1986-08-01), Kricka et al.
Kricka Larry J.
O'Toole Angela M.
Thorpe Gary H. G. H.
Whitehead Thomas P.
Herald William J.
Kepplinger Esther M.
National Research Development Corporation
LandOfFree
Enhanced luminescent or luminometric assay does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Enhanced luminescent or luminometric assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enhanced luminescent or luminometric assay will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-231220