Enhanced in vitro synthesis of active proteins containing...

Details Enhanced in vitro synthesis of active proteins containing... Enhanced in vitro synthesis of active proteins containing...

2006-05-09

2006-05-09

Kam, Chih-Min (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

C435S069100, C435S025000, C435S189000, C435S215000, C435S243000, C530S350000

Reexamination Certificate

active

07041479

ABSTRACT:
Compositions and methods are provided for the enhanced in vitro synthesis of polypeptides containing disulfide bonds. In order to improve the performance of in vitro protein synthesis reactions, pre-treatment and redox buffering of the reaction mix is performed in order to optimize the redox potential. Exogenous enzymes that enhance protein folding and disulfide bond formation may also be added to the reaction.

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