Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2006-12-05
2006-12-05
Qian, Celine (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S476000, C435S463000, C435S468000, C435S455000, C435S254200, C435S252300, C435S320100
Reexamination Certificate
active
07144734
ABSTRACT:
Disclosed herein are methods for generating recombinant DNA molecules in cells using homologous recombination mediated by recombinases and similar proteins. The methods promote high efficiency homologous recombination in bacterial cells, and in eukaryotic cells such as mammalian cells. The methods are useful for cloning, the generation of transgenic and knockout animals, and gene replacement. The methods are also useful for subcloning large DNA fragments without the need for restriction enzymes. The methods are also useful for repairing single or multiple base mutations to wild type or creating specific mutations in the genome. Also disclosed are bacterial strains and vectors which are useful for high-efficiency homologous recombination.
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Copeland Neal G.
Court Donald L.
Ellis Hilary M.
Jenkins Nancy A.
Lee E-Chiang
Dunston Jennifer
Klarquist & Sparkman, LLP
Qian Celine
The United States of America as represented by the Department of
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