Enhanced expression in a plant plastid

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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4353201, 435 691, 4352404, 4351723, 800DIG40, 800DIG43, A01H 400, C12N 504, C12N 1582

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055458173

ABSTRACT:
Novel compositions and methods useful for genetic engineering of plant cells to provide increased expression in the plastids of a plant or plant cell of a protein which produces a phenotype which is present when the plant or plant cell is grown in the absence of means for selecting transformed cells. Expression of the Bacillus thuringiensis bacterial protoxin in a plant chloroplast is exemplified.

REFERENCES:
Morton, B., "Chloroplast DNA Codon Use: Evidence for Selection at the psb A Locus Based on tRNA Availability" Journal of Molecular Evolution 37:273-280 (1993).
Adang, et al., "The Reconstruction and Expression of a Bacillus thuringiensis cryIIIA Gene in Protoplasts and Potato Plants" Plant Molecular Biology 1993 vol. 21 1131-1145.
Bendich, Arnold J. "Why Do Chloroplasts and Mitochondria Contain so Many Copies of their Genome" Bioessays 1987, vol. 6, No. 6 279-282.
Benton, et al., "Signal-Mediated Import of Bacteriophage T7 RNA Polymerase into the Saccharomyces cerevisae Nucleus and Specific Transcription of Target Genes" Molecular and Cell Biology 1990 vol. 10 No 1 353-360.
Blowers, et al "Studies on Chlamydomonas Chloroplast Transformation: Foreign DNA Can be Stably Maintained in the Chromosome" The Plant Cell 1989 vol. 1 123-132.
Boynton, et al. "Chloroplast Transformation in Chlamydomonas with High Velocity Microprojectiles" Science, 1988 vol. 240: 1534-1538.
Boynton, et al. "Manipulating the Chloroplast Genome of Chlamydomonas-Molecular Genetics and Transformation" Current Research in Photosynthesis Proceedings of the VIIth International Conference on Photosynthesis, Stockholm, Sweden, Aug. 6-11, 1989 vol. III 12:509-12: 516.
Daniell, et al. "Transient Foreign Gene Expression of Chloroplasts of Cultured Tobacco Cells After Biolistic Delivery of Chloroplast Vectors" Proc. Natl. Acad. Sci. USA 1990 vol. 87 88-92.
De Block, et al., "Chloroplast Transformation by Agrobacterium tumefaciens" The EMBO Journal 1985 vol. 4 No. 6 1367-1372.
Dunn, et al. "Targeting Bacteriophage T7 RNA Polymerase to the Mammalian Cell Nucleus" Gene 1988 vol. 68 259-266.
Fejes, et al. "Extensive Homologous Chloroplast DNA Recombination in the pt14 Nicotania Somatic Hybrid"; Theor of Appl Genet 1990 vol. 79 28-32.
Golds, et al. "Stable Plastid Transformation in PEG-Treated Protoplasts of Nicotania tabacum" Biotechnology 1993, vol. 11 95-97.
Goldschmidt-Clermont, et al. "Transgenic Expression of Aminoglycoside Adenine Transferase in the Chloroplast: A Selectable Marker for Site-Directed Transformation of Chlamydomonas" Nucleic Acids Research 1991 vol. 19, No. 15 4083-4089.
Gruissem, et al. "Control Mechanisms of Plastid Gene Expression" Critical Reviews in Plant Sciences 1993 vol. 12 1/2 19-55.
Hiratsuka, et al. "The Complete Sequence of the Rice (Oryza sativa) Chloroplast Genome: Intermolecular Recombination Between Distinct tRNA Genes Accounts for a Major Plastid DNA Inversion During the Evolution of Cereals" Mol. Gen. Genet. 1989 vol. 217 185-194.
Lassner, et al. "Targeting of T7 RNA Polymerase in Tobacco Nuclei Mediated by an SV40 Nuclear Location Signal" Plant Molecular Biology 1991 vol. 17 229-234.
Lieber, et al. "High Level Gene Expression in Mammalian Cells by a Nuclear T7-phage RNA Polymerase" Nucleic Acids Research 1989 vol. 7 No. 21 8485-8493.
Maliga, Pal "Towards Plastid Transformation in Flowering Plants" Tibtech, 1993 vol. II 101-107.
Masters, et al., "Yeast Mitochondrial RNA is Homologous to Those Encoded by Bacteriophages T3 and T7" Cell, Oct. 9, 1987 vol. 51 89-99.
McGraw, et al. "Sequence and Analysis of the Gene for Bacteriophage T3 RNA Polymerase" Nucleic Acids Research 1985 vol. 13 No. 18 6753-6767.
Moll, et al. "Streptomycin and Lincomycin Resistances are Selective Plastid Markers in Cultured Nicotania Cells" Mol. Gen. Genet 1990 vol. 221 245-250.
Ohyama, et al. "Chloroplast Gene Organization Deduced from Complete Sequence of Liverwort Marchantia polymorpha Chloroplast DNA" Nature 1986, vol. 322 572-574.
O'Neal, et al. "Isolation of Tobacco SSU Genes: Characterization of a Transcriptionally Active Pseudogene" Nucleic Acids Research 1987 vol. 15, No. 21 8661-8677.
Palmer, Jeffrey D. "Contrasting Modes and Tempos of Genome Evolution in Land Plant Organelles" TIG 1990 vol. 6, No. 4 115-120.
Perlak, et al., "Modification of the Coding Sequence Enhances Plant Expression of Insect Control Protein Genes" Proc. Natl. Acad. Sci. 1991 vol. 88 3324-3328.
Petit, Charles "The Chloroplast Genome--An Essential Intruder" Mosaic 1991, vol. 22 No. 3 36-45.
Rosenberg, et al. "Vectors for Selective Expression of Cloned DNA's by T7 RNA Polymerase" Gene 1987 vol. 56 125-135.
Schinkel, et al. "Mitochondrial RNA Polymerase: Dual Role in Transcription and Replication" TIG 1989 vol. 5, No. 5 149-153.
Shinozaki, et al., "The Complete Nucleotide Sequence of the Tobacco Chloroplast Genome: its Gene Organization and Expression" The EMBO Journal 1986 vol. 5 No. 9 2043-2049.
Staub, et al. "Long Regions of Homologous DNA are Incorporated into the Tobacco Plastid Genome by Transformation" The Plant Cell, 1992 vol. 4; 39-45.
Staub, et al. "Accumulation of D1 Polypeptide in Tobacco Plastids is Regulated via the Untranslated Region of the psbA mRNA" The EMBO Journal, 1993 vol. 12 601-606.
Svab, et al. "Efficient Plastid Transformation in Tobacco by Selection of a Chimeric aaDa Gene". Crop Improvement via Biotechnology; an International Perspective 208 (1991).
Svab, et al. "High-Frequency Plastid Transformation in Tobacco by Selection for a Chimeric aadA Gene" Proc. Natl. Acad. Sci, USA 1993 vol. 90 913-917.
Svab, et al. "Stable Transformation of Plastids in Higher Plants" Proc.Natl. Acad. Sci, USA 1990 vol. 87 8526-8530.
Svab, et al. "Mutation Proximal to the tRNA Binding Region of the Nicotania Plastid 16S rRNA Confers Resistance to Spectinomycin" Mol. Gen. Genet. 1991 vol. 228 316-319.
Svab, et al. "Aminoglycoside-3" Adenyltransferase Confers Resistance to Spectinomycin and Streptomycin in Nicotania tabacum" Plant Molecular Biology 1990 vol. 14 197-205.
Vaeck, et al., "Transgenic Plants Protected from Insect Attack", Nature 1987, vol. 328 33-37.
Wong, et al., "Arabidopsis thaliana Small Subunit Leader and Transit Peptide Enhance the Expression of Bacillus thuringiensis Proteins in Transgenic Plants" Plant Molecular Biology 1992 vol. 20 81-93.
Maliga, et al. (1993) Phil. Trans. R. Soc. Fond. B 342:203-208.
Shimada et al (1991) Nucleic Acids Research 19(5):983-995.
Chungjatupornchai, et al., "Expression of the Mosquitocidal-Protein Genes of Bacillus thuringensis-SSP-israelensis and the Herbicide-Resistance Gene Bar in synechocystis PCC6803". Current Microbiology vol. 2 (1990), pp. 283-288.
Carrer, et al. "Kanamycin resistance as a Selectable Marker for Plastid Transformation in tobacco." Molecular General Genetics (1993) 241:49-56.
McBride, et al., "Controlled Expression of Plastid transgenes in Plants Based on a Nuclear DNA-Encoded and Plastid-Targeted T7 RNA Polymerase" Proceeddings of the National Academy of Sciences of USA vol. 91, (1994) pp. 7301-7305.

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