Enhanced detection of RNA using a panel of truncated...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

Reexamination Certificate

active

07981616

ABSTRACT:
The present invention provides truncated gene-specific primers in panels that can be used during the reverse transcription step of RT-PCR to increase signal detection of cancer gene markers in a tissue sample. Also provided are forward and reverse primers for RT-PCR. Methods of using the primers are also provided.

REFERENCES:
patent: 5314809 (1994-05-01), Erlich et al.
patent: 5773213 (1998-06-01), Gullans et al.
patent: 5801016 (1998-09-01), Morioka et al.
patent: 5814491 (1998-09-01), Vijg et al.
patent: 5837442 (1998-11-01), Tsang
patent: 5843761 (1998-12-01), Barnett et al.
patent: 5854033 (1998-12-01), Lizardi
patent: 5882856 (1999-03-01), Shuber
patent: 5958349 (1999-09-01), Petersen et al.
patent: 6004756 (1999-12-01), Watson et al.
patent: 6037129 (2000-03-01), Cole et al.
patent: 6040138 (2000-03-01), Lockhart et al.
patent: 6057105 (2000-05-01), Hoon et al.
patent: 6183960 (2001-02-01), Lizardi
patent: 6210884 (2001-04-01), Lizardi
patent: 6245517 (2001-06-01), Chen et al.
patent: 6251601 (2001-06-01), Bao et al.
patent: 6261776 (2001-07-01), Pirrung et al.
patent: 6306643 (2001-10-01), Gentalen et al.
patent: 6309823 (2001-10-01), Cronin et al.
patent: 6344329 (2002-02-01), Lizardi
patent: 6346413 (2002-02-01), Fodor et al.
patent: 6374684 (2002-04-01), Dority et al.
patent: 6403037 (2002-06-01), Chang et al.
patent: 6406844 (2002-06-01), Pirrung et al.
patent: 6416952 (2002-07-01), Pirrung et al.
patent: 6431476 (2002-08-01), Taylor et al.
patent: 6440725 (2002-08-01), Pourahmadi et al.
patent: 7101663 (2006-09-01), Godfrey et al.
patent: 7824857 (2010-11-01), Mitas et al.
patent: 1 050 587 (2000-11-01), None
patent: WO 98/08970 (1990-08-01), None
patent: WO 95/11687 (1995-05-01), None
patent: WO 99/13104 (1999-03-01), None
patent: WO 00/44774 (2000-08-01), None
patent: WO 00/72970 (2000-12-01), None
patent: WO 00/73412 (2000-12-01), None
patent: WO 00/73413 (2000-12-01), None
patent: WO 01/01129 (2001-01-01), None
patent: WO 01/45845 (2001-06-01), None
patent: WO 01/57253 (2001-08-01), None
patent: WO 01/84463 (2001-11-01), None
patent: WO 02/18902 (2002-03-01), None
patent: WO 02/052030 (2002-07-01), None
patent: WO 02/070751 (2002-09-01), None
patent: WO 03/055973 (2002-12-01), None
patent: WO 03/072253 (2003-09-01), None
patent: WO 03/077055 (2003-09-01), None
patent: WO 2004/048931 (2004-06-01), None
Schwarz et al. (J Virol Methods. Feb. 1995;51(2-3):349-56).
Cerveira et al. (Br J Haematol. Jun. 2000;109(3):638-40).
Mitas et al. (Br J Cancer. Mar. 18, 2002;86(6):899-904).
Baker, MK, et al., “Molecular detection of breast cancer cells in the peripheral blood of advanced-stage breast cancer patients using multimarker real-time reverse transcription-polymerase chain reaction and a novel porous barrier density gradient centrifugation technology,”Clin Cancer Res9:4865-4871 (2003).
Bieche, I, et al., “Novel approach to quantitative polymerase chain reaction using real-time detection: application to the detection of gene amplification in breast cancer,”Int J Cancer, 78:661-666 (1998).
Bingle, CD, et al., Characterisation of the humanpluncgene, a gene product with an upper airways and nasopharyngeal restricted expression pattern,Biochim Biophys Acta, 1493:363-367 (2000).
Coombs, NJ, et al., “Optimisation of DNA and RNA extraction from archival formalin-fixed tissue,”Nucleic Acids Res27:e12 (i-iii) (1999).
Corey, E, et al., “Detecticin of disseminated prostate cells by reverse transcription-polymerase chain reaction (RT-PCR): technical and clinical aspects,”Int J Cancer77:655-673 (1998).
Czygan, M., et al., “Boma disease virus in human brains with a rare form of hippocampal degeneration but not in brains of patients with common neuropsychiatric disorders,”J Infec Dis, 180(5):1695-1699 (1999).
Database Genbank, National Center for Biotechnology Information NLM, N IH; Accession No. AJ246858.1 (1999).
Dennis, P, et al., “Monitoring gene expression in mixed microbial communities by using DNA microarrays,”Appl Environ Microbiol69:769-778 (2003).
Ghadersohi A, et al., “Prostate Epithelium-derived Ets Transcription Factor mRNA Is Overexpressed in Human Breast Tumors and Is a Candidate Breast Tumor Marker and a Breast Tumor Antigen,”Clin Cancer Res, 7:2731-2738 (2001).
Godfrey, TE, et al., “Quantitative mRNA expression analysis from formalin-fixed, paraffin-embedded tissues using 5′ nuclease quantitative reverse transcription-polymerase chain reaction,”J Mol Diagn2:84-91 (2000).
Goldsworthy, SM, et al., “Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue,”Mol Carcinog25:86-91 (1999).
Körbler, T, et al., “A simple method for RNA isolation from formalin-fixed and paraffin-embedded lymphatic tissues,”Exp Mol Pathol74:336-340 (2003).
Masuda, N, et al., “Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples,”Nucleic Acids Res27:4436-4443 (1999).
Mitas, M, et al., “Lunxis a superior molecular marker for detection of non-small lung cell cancer in peripheral blood,”J Mol Diagn5:237-242 (2003).
Mitas, M, et al., “Prostate-Specific Ets (PSE) factor: a novel marker for detection of metastatic breast cancer in axillary lymph nodes,”Br J Cancer86:899-904 (2002).
Mitas, M, et al., “Quantitative real-time RT-PCR detection of breast cancer micrometastasis using a multigene marker panel,”Int J Cancer93:162-171 (2001).
Mitas, M, et al., “Real-time reverse transcription-PCR detectsKS1/4mRNA in mediastinal lymph nodes from patients with non-small cell lung cancer,”Clin Chem49:312-315 (2003).
Muller, PY, et al., “Processing of gene expression data generated by quantitative real-time RT-PCR,”Biotechniques, 32:1372-1374, 1376, 1378-1379 (2002).
Oettgen, P, et al., “PDEF, a novel prostate epithelium-specific Ets transcription factor, interacts with the androgen receptor and activates prostate-specific antigen gene expression,”J Biol Chem, 275:1216-1225 (2000).
Perez, MS, et al., “Isolation and characterization of a cDNA encoding the KS1/4 epithelial carcinoma marker,”J. Immunol, 142:3662-3667 (1989).
Rosenberg, R, et al., “Comparison of Two Density Gradient Centrifugation Systems for the Enrichment of Disseminated Tumor Cells in Blood,”Cytometry, 49:150-158 (2002).
Specht, K, et al., “Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue,”Am J Pathol158:419-429 (2001).
Stanta, G, et al., “RNA extracted from paraffin-embedded human tissues is amenable to analysis by PCR amplification,”Biotechniques11:304, 306, 308 (1991).
Sung, YK, et al., “Plunc, a member of the secretory gland protein family, is up-regulated in nasal respiratory epithelium after olfactory bulbectomy,”J Biol Chem, 277:12762-12769 (2002).
Weston, W, et al., “Differential display identification ofplunc, a novel gene expressed in embryonic palate, nasal epithelium, and adult lung,”J Biol Chem, 274:13698-13703 (1999).
“Genecard for protein-coding TACSTD1 GC02P047449: tumor-associated calcium signal,” GeneCards, pp. 1-9 (2006).
“Genecard for protein-coding TACSTD1 GC02P047449: tumor-associated calcium signal,” GeneCards, pp. 1-6 (2007 update).
“TagMan® One-Step RT-PCR Master Mix Reagents Kit: Protocol,” Biosystems, pp. 1-26 (1999).
Abba et al., “Gene Expression Signature of Estrogen Receptor α Status in Breast Cancer,” BMC Genomics, 6:37:1-13 2005.
ABI PRISM 7700 Sequence Detection System, User Bulletin #5, “Multiplex PCR with TagMan® VIC Probes,” Applied Biosystems, pp. 1-20 (1998; Updated 2001).
Aronow et al., “Microarray Analysis of Trophoblast Differentiation: Gene Expression Reprogramming in Key Gene Function Categories,”

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Enhanced detection of RNA using a panel of truncated... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enhanced detection of RNA using a panel of truncated..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enhanced detection of RNA using a panel of truncated... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2672806

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.