Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase – Separation of immune complex from unbound antigen or antibody
Patent
1996-07-12
1998-12-29
Chin, Christopher L.
Chemistry: analytical and immunological testing
Involving immune complex formed in liquid phase
Separation of immune complex from unbound antigen or antibody
435 71, 435 78, 435 793, 435 794, 435 795, 435807, 436538, 436545, 436161, 436162, 436804, 73 1902, 73 2322, 73 6152, G01N 33533
Patent
active
058540849
ABSTRACT:
A method for detecting an analyte of interest present in a mixture at an ultralow concentration includes selecting a radioactive derivatizing agent comprising a multiphoton-emitting radioisotope moiety and a moiety reactive with the analyte of interest, the radioisotope moeity being bound to the derivatizing agent by a bond that is stable under the conditions employed in the other steps of the method, derivatizing the analyte of interest with the derivatizing agent, separating the analyte of interest from other components of the mixture by chromatography, and detecting the analyte of interest using multiphoton detection. The derivatizing step may be performed before or after fractionation. A radiophore for multiphoton emission enhanced chromatography has a first moeity bound to a multiphoton-emitting radioisotope, and a second moiety that is reactive with a functional group of an analyte of interest. The first moiety may be a benzene group having an alkoxy, amino, or thiol group, bound to a halogen radioisotope; a complexing agent able to form a stable complex with a metal radioisotope such as a lanthanide or heavy metal.
REFERENCES:
patent: 5532122 (1996-07-01), Drukier
Gosling, James P., Clinical Chemistry, 36/8, 1408-1427, 1990.
Sack et al., Analytical Chemistry, 57, 1290-1295, 1985.
Voet et al., Biochemistry, Second Edition, 71-103, 1995.
Bielski Roman
Drukier Andrzej K.
BioTraces, Inc.
Chin Christopher L.
Nguyen Bao-Thuy L.
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